Abstract
The linker histone H1 is a key player in chromatin organization, yet our understanding of the regulation of H1 functions by post-translational modifications is very limited. We provide here the first functional characterization of H1 acetylation. We show that H1.4K34 acetylation (H1.4K34ac) is mediated by GCN5 and is preferentially enriched at promoters of active genes, where it stimulates transcription by increasing H1 mobility and recruiting a general transcription factor. H1.4K34ac is dynamic during spermatogenesis and marks undifferentiated cells such as induced pluripotent stem (iPS) cells and testicular germ cell tumors. We propose a model for H1.4K34ac as a novel regulator of chromatin function with a dual role in transcriptional activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Amino Acid Sequence
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Cell Cycle / genetics
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Gene Expression Regulation, Neoplastic
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Histone Acetyltransferases
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Histones / genetics
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Histones / metabolism*
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Humans
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Lysine / genetics
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Lysine / metabolism*
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Male
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Molecular Sequence Data
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Pluripotent Stem Cells / metabolism
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Promoter Regions, Genetic
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Seminoma / genetics
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Seminoma / metabolism
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Spermatogenesis / genetics
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TATA-Binding Protein Associated Factors / metabolism
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Testicular Neoplasms / genetics
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Testicular Neoplasms / metabolism
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Transcription Factor TFIID / metabolism
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Transcription Initiation Site
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Transcriptional Activation*
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Up-Regulation
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p300-CBP Transcription Factors / metabolism*
Substances
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Histones
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TATA-Binding Protein Associated Factors
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Transcription Factor TFIID
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Histone Acetyltransferases
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p300-CBP Transcription Factors
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p300-CBP-associated factor
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TATA-binding protein associated factor 250 kDa
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Lysine