Format

Send to:

Choose Destination
See comment in PubMed Commons below
PLoS Genet. 2011 Dec;7(12):e1002380. doi: 10.1371/journal.pgen.1002380. Epub 2011 Dec 15.

A high-resolution whole-genome map of key chromatin modifications in the adult Drosophila melanogaster.

Author information

  • 1Yale Stem Cell Center and Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut, United States of America.

Abstract

Epigenetic research has been focused on cell-type-specific regulation; less is known about common features of epigenetic programming shared by diverse cell types within an organism. Here, we report a modified method for chromatin immunoprecipitation and deep sequencing (ChIP-Seq) and its use to construct a high-resolution map of the Drosophila melanogaster key histone marks, heterochromatin protein 1a (HP1a) and RNA polymerase II (polII). These factors are mapped at 50-bp resolution genome-wide and at 5-bp resolution for regulatory sequences of genes, which reveals fundamental features of chromatin modification landscape shared by major adult Drosophila cell types: the enrichment of both heterochromatic and euchromatic marks in transposons and repetitive sequences, the accumulation of HP1a at transcription start sites with stalled polII, the signatures of histone code and polII level/position around the transcriptional start sites that predict both the mRNA level and functionality of genes, and the enrichment of elongating polII within exons at splicing junctions. These features, likely conserved among diverse epigenomes, reveal general strategies for chromatin modifications.

PMID:
22194694
[PubMed - indexed for MEDLINE]
PMCID:
PMC3240582
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk