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Nano Lett. 2011 Oct 12;11(10):4074-8. doi: 10.1021/nl201225r. Epub 2011 Sep 13.

Two-photon 3D FIONA of individual quantum dots in an aqueous environment.

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  • 1Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.

Abstract

We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9 × 9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.

PMID:
21882883
[PubMed - indexed for MEDLINE]
PMCID:
PMC3192306
Free PMC Article

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