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J Neuroimmunol. 2011 May;234(1-2):131-40. doi: 10.1016/j.jneuroim.2011.02.001. Epub 2011 Mar 11.

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

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  • 1Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA.

Abstract

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
21397340
[PubMed - indexed for MEDLINE]
PMCID:
PMC3092854
Free PMC Article

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