Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anal Biochem. 2011 Apr 1;411(1):64-70. doi: 10.1016/j.ab.2010.12.028. Epub 2010 Dec 24.

Whole gene amplification and protein separation from a few cells.

Author information

  • 1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

Abstract

Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same cohort of cyanobacterial cells. This technology opens avenues for studying protein profiles of precious environmental microbial samples and simultaneously accessing genomic information based on WGA.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:
21185803
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk