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J Microsc. 2011 Feb;241(2):119-24. doi: 10.1111/j.1365-2818.2010.03419.x.

Enhancing collection efficiency in large field of view multiphoton microscopy.

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  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.

Abstract

Many multiphoton imaging applications would benefit from a larger field of view; however, large field of views (>mm) require low magnification objectives which have low light collection efficiencies. We demonstrate a light collection system mounted on a low magnification objective that increases fluorescence collection by as much as 20-fold in scattering tissues. This peripheral detector results in an effective numerical aperture of collection >0.8 with a 3-4 mm field of view.

© 2010 The Authors Journal compilation © 2010 The Royal Microscopical Society.

PMID:
21118215
[PubMed - indexed for MEDLINE]
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