Format

Send to:

Choose Destination
See comment in PubMed Commons below
RNA. 2010 Dec;16(12):2564-9. doi: 10.1261/rna.2350710. Epub 2010 Oct 26.

High-efficiency siRNA-based gene knockdown in human embryonic stem cells.

Author information

  • 1Yale Stem Cell Center, New Haven, Connecticut 06520, USA.

Abstract

Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown.

PMID:
20978109
[PubMed - indexed for MEDLINE]
PMCID:
PMC2995416
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk