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Biosens Bioelectron. 2011 Jan 15;26(5):2707-10. doi: 10.1016/j.bios.2010.09.006. Epub 2010 Sep 15.

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.

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  • 1Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, 51 Blossom St., Boston, MA, USA. konry.tania@gmail.com

Abstract

Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.

Copyright © 2010 Elsevier B.V. All rights reserved.

PMID:
20888750
[PubMed - indexed for MEDLINE]
PMCID:
PMC3141325
Free PMC Article
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