Send to

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2010;478:541-62. doi: 10.1016/S0076-6879(10)78026-5.

Metabolic labeling of glycoconjugates with photocrosslinking sugars.

Author information

  • 1Division of Translational Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, USA.


Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.

Copyright (c) 2010 Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk