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Dig Liver Dis. 2010 Oct;42(10):679-84. doi: 10.1016/j.dld.2010.02.003. Epub 2010 Mar 15.

Tissue microarray constructs to predict a response to chemoradiation in rectal cancer.

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  • 1Department of Surgery, University of Texas Southwestern Medical Center, United States. Sergio.Huerta@UTSouthwestern.edu

Abstract

PURPOSE:

To identify, using tissue microarray (TMA), an immunohistochemical panel predictive of response to ionizing radiation (IR) in rectal cancer.

METHODS:

TMA constructs were prepared from archived stage II/III rectal tumors and matching adjacent mucosa (n=38) from patients treated with pre-operative chemoradiation. Immunohistochemistry (IHC) was performed for MIB, Cyclin E, p21, p27, p53, survivin, Bcl-2, and BAX. Immunoreactivity along with clinical variables was subjected to univariate and forward stepwise logistic regression analyses.

RESULTS:

Pathological complete response (pCR) was 23.9%. The number of positive lymph nodes obtained in the resected specimen was associated with pCR. Immunoreactivity for MIB (Sn 15%, Sp 65%, OR 0.33), p53 (Sn 3%, Sp 84%, OR 0.16), Bcl-2 (Sn 11%, Sp 74%, OR 0.35), and BAX (Sn 92%, Sp 80%, OR 46) was associated with pathological response (all p's<0.001). Forward stepwise logistic regression analysis demonstrated that MIB was an independent predictor of a response to chemoradiation (p=0.001).

CONCLUSIONS:

A combined panel of mediators of apoptosis alone or combined with clinical factors is a feasible approach that can be applied to rectal tumor biopsies to predict a response to chemoradiation. The most sensitive factor was BAX; while MIB independently predicted a response to chemoradiation.

Published by Elsevier Ltd.

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PMID:
20227932
[PubMed - indexed for MEDLINE]
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