Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2009;457:373-93. doi: 10.1016/S0076-6879(09)05021-6.

Assessment of in vivo mitochondrial metabolism by magnetic resonance spectroscopy.

Author information

  • 1Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

Magnetic resonance spectroscopy (MRS), a companion technique to the more familiar MRI scan, has emerged as a powerful technique for studying metabolism noninvasively in a variety of tissues. In this article, we review two techniques that we have developed which take advantage of the unique characteristics of (31)P and (13)C MRS to investigate two distinct parameters of muscle mitochondrial metabolism; ATP production can be estimated by using the (31)P saturation-transfer technique, and oxidation via the TCA cycle can be modeled from (13)C MRS data obtained during the metabolism of a (13)C-labeled substrate. We will also examine applications of the techniques to investigate how these parameters of muscle mitochondrial metabolism are modulated in insulin resistant and endurance trained individuals.

PMID:
19426879
[PubMed - indexed for MEDLINE]
PMCID:
PMC3077057
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk