Format

Send to:

Choose Destination
See comment in PubMed Commons below
Yale J Biol Med. 2005 Jan;78(1):25-36.

Apoptosis role of FAS/FAS ligand system in the regulation of myelopoiesis.

Author information

  • 1Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, Dammam, Saudi Arabia. falenzi@kfu.edu.sa

Abstract

OBJECTIVES:

CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis.

METHODS:

Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony assay and the proliferative capacity of CFU-GM was measured by replating the primary colony and observing the secondary colony formation. Human marrow cells were treated with IETD (caspase-8 inhibitor) or anti-Fas CH-11 Mab.

RESULTS:

Treatment with the CFU-GM with IETD significantly increased, the proliferative capacity, while anti-Fas CH-11 Mab markedly reduced it. Fas and FasL expression were demonstrated using RT-PCR and immunofluorescence respectively.

CONCLUSION:

Fas, FasL, and caspase activation are likely to play an important role in the regulation of myelopoiesis.

PMID:
16197727
[PubMed - indexed for MEDLINE]
PMCID:
PMC2259140
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk