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J Neuroimmunol. 2001 Aug 30;118(2):194-202.

In vitro evidence that immunuaffinity-purified MOG contains immunogenic quantities of contaminating mouse IgG; techniques for producing Ig-free MOG.

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  • 1Center for Neurologic Diseases, Laboratory of Molecular Immunology, Brigham and Women's Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, HIM 786, Boston, MA 02115, USA.

Abstract

Myelin oligodendrocyte glycoprotein (MOG) has attracted considerable attention due to its possible role in multiple sclerosis (MS). Previous studies have shown that MOG-reactive T cells are more commonly detected in MS patients than controls. In this report, we studied human MOG-reactive T cell clones generated from healthy individuals as well as MS patients. Our results indicate that immunoaffinity-purified MOG, which was routinely used in prior studies, is contaminated by anti-MOG antibody (mouse IgG), despite the inability to detect IgG by Western blotting. Here, we used continuous SDS-PAGE, which enabled us to isolate highly purified MOG devoid of contaminating mouse IgG.

PMID:
11498254
[PubMed - indexed for MEDLINE]
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