Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH.
Körfer G, Novoa C, Kern J, Balla E, Grütering C, Davari MD, Martinez R, Vojcic L, Schwaneberg U.
Körfer G, et al.
Appl Microbiol Biotechnol. 2018 Nov;102(22):9607-9620. doi: 10.1007/s00253-018-9308-7. Epub 2018 Aug 23.
Appl Microbiol Biotechnol. 2018.
PMID: 30141080
In this manuscript, we report a directed evolution campaign of the highly active bacterial phytase from Yersinia mollaretii (YmPh) towards a broadened pH activity spectrum. ...Kinetic characterization of the improved variants, YmPh-M10 and -M16, showed up to sevenfo …
In this manuscript, we report a directed evolution campaign of the highly active bacterial phytase from Yersinia mollaretii (Y …