Construction and expression of a polycistronic plasmid encoding N-acetylglucosamine 2-epimerase and N-acetylneuraminic acid lyase simultaneously for production of N-acetylneuraminic acid.
Sun W, Ji W, Li N, Tong P, Cheng J, He Y, Chen Y, Chen X, Wu J, Ouyang P, Xie J, Ying H.
Sun W, et al.
Bioresour Technol. 2013 Feb;130:23-9. doi: 10.1016/j.biortech.2012.12.042. Epub 2012 Dec 19.
Bioresour Technol. 2013.
PMID: 23280182
Nal from Escherichia coli K12 and AGEs from Synechocystis sp. PCC 6803 (snAGE) and Anabaena sp. CH1 (anAGE) were used. And four polycistronic plasmids were constructed in which the positions of AGE gene differed with respect to Nal gene. ...
Nal from Escherichia coli K12 and AGEs from Synechocystis sp. PCC 6803 (snAGE) and Anabaena sp. CH1 (anAGE) were used. …