Format

Send to

Choose Destination

Links from PubMed

See comment in PubMed Commons below
Asian Pac J Trop Biomed. 2013 Mar;3(3):174-81. doi: 10.1016/S2221-1691(13)60045-3.

Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats.

Author information

  • 1Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt.

Abstract

OBJECTIVE:

To evaluate the effect of mesenchymal stem cells (MSCs) in rats with anti-Thy1,1 nephritis.

METHODS:

Female albino rats were divided into three groups, control group, anti-Thy1,1 group and treatment with i.v. MSCs group. MSCs were derived from bone marrow of male albino rats, Y-chromosome gene was detected by polymerase chain reaction in the kidney. Serum urea and creatinine were estimated for all groups. Kidney of all studied groups was examined histologically and histochemically (total carbohydrates and total proteins). DNA fragmentation and expression of α-SMA were detected.

RESULTS:

Kidney of animals injected with anti-Thy1,1 showed inflammatory leucocytic infiltration, hypertrophied glomeruli, tubular necrosis and congestion in the renal blood vessels. The kidney tissue also showed reduction of carbohydrates and total proteins together with increase in apoptosis and in expression of α-SMA. Moreover, the levels of urea and creatinine were elevated. Treating animals with MSCs revealed that kidney tissue displayed an improvement in the histological and histochemical changes. Apoptosis and α-SMA expression were decreased, and the levels of urea and creatinine decreased.

CONCLUSIONS:

The obtained results demonstrated the potential of MSCs to ameliorate the structure and function of the kidney in rats with anti-Thy1,1 nephritis possibly through the release of paracrine growth factor(s).

KEYWORDS:

Anti-Thy1,1; Apoptosis; Mesenchymal stem cells; Nephritis; α-SMA

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk