Format

Send to

Choose Destination

Links from PubMed

See comment in PubMed Commons below
Arch Facial Plast Surg. 2011 May-Jun;13(3):185-9. doi: 10.1001/archfacial.2010.115. Epub 2011 Jan 17.

Evaluating revascularization and flap survival using vascular endothelial growth factor in an irradiated rat model.

Author information

  • 1Department of Otolaryngology-Head and Neck Surgery, Oregon Health & Science University, Mail Code PV01, 3181 SW Sam Jackson Park Rd, Portland, OR 29239, USA. angelosp@ohsu.edu

Abstract

OBJECTIVE:

To evaluate the role of vascular endothelial growth factor (VEGF) plasmid DNA (pDNA) in improving flap revascularization in a previously developed rat model. Our hypothesis was that the uptake and expression of VEGF pDNA in the wound bed would improve revascularization and flap viability.

DESIGN:

Twenty-eight male Sprague-Dawley rats received a total dose of 40 Gy electron beam radiation to the ventral abdominal wall. After a recovery period, they underwent a ventral fasciocutaneous flap procedure with a 2-hour ischemia period. Group 1 (n = 14) received topical VEGF pDNA, in vivo cationic polymer, and fibrin sealant. Group 2 (n = 14) received topical cationic polymer and fibrin sealant only. Seven of the rats from each group underwent pedicle ligation at 8 or 14 days. The primary outcome measure was percentage of flap revascularization 5 days after pedicle ligation.

RESULTS:

Rats receiving VEGF pDNA had a significantly higher rate of flap revascularization (90.8% vs 79.8%) after pedicle ligation at 14 days (P = .045). At 8 days, rats receiving VEGF pDNA (group 1) had an increased rate of flap revascularization (58.2% vs 42.8%) that approached significance (P = .11).

CONCLUSION:

This study demonstrates the potential of VEGF pDNA to improve revascularization and flap viability in previously irradiated tissue.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems
    Loading ...
    Write to the Help Desk