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Nucleic Acids Res. 2010 May;38(8):2603-6. doi: 10.1093/nar/gkq143. Epub 2010 Mar 8.

GeneDesign 3.0 is an updated synthetic biology toolkit.

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  • 1McKusick-Nathans Institute of Genetic Medicine, High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA. notadoctor@jhmi.edu


GeneDesign is a set of web applications that provides public access to a nucleotide manipulation pipeline for synthetic biology. The server is public and freely accessible, and the source is available for download under the New BSD License. Since GeneDesign was published and made publicly available 3 years ago, we have made its code base more efficient, added several algorithms and modules, updated the restriction enzyme library, added batch processing capabilities, and added several command line modules, all of which we briefly describe here.

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