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Opt Lett. 1998 Aug 1;23(15):1221-3.

Three-dimensional microscopy with phase-shifting digital holography.

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  • 1Graduate Course of Science and Engineering, Saitama University, Urawa, Saitama 338-8570, Japan.


We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

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