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Results: 1 to 20 of 61

1.

A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification.

Kang K, Yang K, Zhong J, Tian Y, Zhang L, Zhai J, Zhang L, Song C, Gou CY, Luo J, Gou D.

J Anim Sci Biotechnol. 2014 Oct 2;5(1):45. doi: 10.1186/2049-1891-5-45. eCollection 2014.

PMID:
25324970
[PubMed]
Free PMC Article
2.

Gene Assembly from Chip-Synthesized Oligonucleotides.

Eroshenko N, Kosuri S, Marblestone AH, Conway N, Church GM.

Curr Protoc Chem Biol. 2012 Mar 1;2012. pii: ch110190.

PMID:
25077042
[PubMed]
Free PMC Article
3.

Polymerase chain reaction optimization for amplification of Guanine-Cytosine rich templates using buccal cell DNA.

Bhagya CH, Wijesundera Sulochana WS, Hemamali NP.

Indian J Hum Genet. 2013 Jan;19(1):78-83. doi: 10.4103/0971-6866.112898.

PMID:
23901197
[PubMed]
Free PMC Article
4.

Inhibitory effects of choline-O-sulfate on amyloid formation of human islet amyloid polypeptide.

Hagihara M, Takei A, Ishii T, Hayashi F, Kubota K, Wakamatsu K, Nameki N.

FEBS Open Bio. 2012 Feb 28;2:20-5. doi: 10.1016/j.fob.2012.02.001. Print 2012.

PMID:
23650576
[PubMed]
Free PMC Article
5.

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky's disease in infected pigs.

Serena MS, Geisler C, Metz GE, Corva SG, Mórtola EC, Larsen A, Jarvis DL, Echeverría MG.

Protein Expr Purif. 2013 Jul;90(1):1-8. doi: 10.1016/j.pep.2013.04.008. Epub 2013 Apr 28.

PMID:
23631926
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

Amplification of GC-rich Putative Mouse PeP Promoter using Betaine and DMSO in Ammonium Sulfate Polymerase Chain Reaction Buffer.

Seifi T, Ghaedi K, Salamian A, Tanhaei S, Safari F, Hojati Z, Tavassoli M, Baharvand H, Esfahani MH.

Avicenna J Med Biotechnol. 2012 Oct;4(4):206-9.

PMID:
23408119
[PubMed]
Free PMC Article
7.

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples.

Zhang EZ, Bartels DJ, Frantz JD, Seepersaud S, Lippke JA, Shames B, Zhou Y, Lin C, Kwong A, Kieffer TL.

Virol J. 2013 Feb 12;10:53. doi: 10.1186/1743-422X-10-53.

PMID:
23402332
[PubMed - indexed for MEDLINE]
Free PMC Article
8.

Homeostasis and catabolism of choline and glycine betaine: lessons from Pseudomonas aeruginosa.

Wargo MJ.

Appl Environ Microbiol. 2013 Apr;79(7):2112-20. doi: 10.1128/AEM.03565-12. Epub 2013 Jan 25. Review.

PMID:
23354714
[PubMed - indexed for MEDLINE]
Free PMC Article
9.

Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers.

Schneider UV, Mikkelsen ND, Lindqvist A, Okkels LM, Jøhnk N, Lisby G.

PLoS One. 2012;7(6):e38451. doi: 10.1371/journal.pone.0038451. Epub 2012 Jun 6.

PMID:
22701644
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

Duplex Detection of TP53 Arg72Pro and 16 bp Del/Ins Polymorphisms by a Simple Optimized PCR-RFLP Method.

Lajin B, Alachkar A, Alhaj SA.

N Am J Med Sci. 2012 May;4(5):212-5. doi: 10.4103/1947-2714.95900.

PMID:
22655279
[PubMed]
Free PMC Article
11.

A capillary electrophoresis sequencing method for the identification of mutations in the inverted terminal repeats of adeno-associated virus.

Mroske C, Rivera H, Ul-Hasan T, Chatterjee S, Wong KK.

Hum Gene Ther Methods. 2012 Apr;23(2):128-36. doi: 10.1089/hgtb.2011.231. Epub 2012 May 21.

PMID:
22612656
[PubMed - indexed for MEDLINE]
Free PMC Article
12.

Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models.

Birdsell DN, Pearson T, Price EP, Hornstra HM, Nera RD, Stone N, Gruendike J, Kaufman EL, Pettus AH, Hurbon AN, Buchhagen JL, Harms NJ, Chanturia G, Gyuranecz M, Wagner DM, Keim PS.

PLoS One. 2012;7(3):e32866. doi: 10.1371/journal.pone.0032866. Epub 2012 Mar 16.

PMID:
22438886
[PubMed - indexed for MEDLINE]
Free PMC Article
13.

Unexpected instability of family of repeats (FR), the critical cis-acting sequence required for EBV latent infection, in EBV-BAC systems.

Kanda T, Shibata S, Saito S, Murata T, Isomura H, Yoshiyama H, Takada K, Tsurumi T.

PLoS One. 2011;6(11):e27758. doi: 10.1371/journal.pone.0027758. Epub 2011 Nov 16.

PMID:
22114684
[PubMed - indexed for MEDLINE]
Free PMC Article
14.

Validation of kinetics similarity in qPCR.

Bar T, Kubista M, Tichopad A.

Nucleic Acids Res. 2012 Feb;40(4):1395-406. doi: 10.1093/nar/gkr778. Epub 2011 Oct 19. Review.

PMID:
22013160
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

Mutagenesis and analysis of genetic mutations in the GC-rich KISS1 receptor sequence identified in humans with reproductive disorders.

Madeira da Silva L, Vandepas L, Bianco SD.

J Vis Exp. 2011 Sep 4;(55):e2897. doi: 10.3791/2897.

PMID:
21912371
[PubMed - indexed for MEDLINE]
Free PMC Article
16.

Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA).

Sun J, Xu J, Liang P, Mao Q, Huang Y, Lv X, Deng C, Liang C, de Hoog GS, Yu X.

Parasit Vectors. 2011 Jun 7;4:98. doi: 10.1186/1756-3305-4-98.

PMID:
21649899
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer.

Horáková H, Polakovičová I, Shaik GM, Eitler J, Bugajev V, Dráberová L, Dráber P.

BMC Biotechnol. 2011 Apr 18;11:41. doi: 10.1186/1472-6750-11-41.

PMID:
21501492
[PubMed - indexed for MEDLINE]
Free PMC Article
18.

Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.

Chen S, Wang F, Beaulieu JC, Stein RE, Ge B.

Appl Environ Microbiol. 2011 Jun;77(12):4008-16. doi: 10.1128/AEM.00354-11. Epub 2011 Apr 15.

PMID:
21498750
[PubMed - indexed for MEDLINE]
Free PMC Article
19.

High-quality DNA sequence capture of 524 disease candidate genes.

Shen P, Wang W, Krishnakumar S, Palm C, Chi AK, Enns GM, Davis RW, Speed TP, Mindrinos MN, Scharfe C.

Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6549-54. doi: 10.1073/pnas.1018981108. Epub 2011 Apr 5.

PMID:
21467225
[PubMed - indexed for MEDLINE]
Free PMC Article
20.

Detecting potentially virulent Vibrio vulnificus strains in raw oysters by quantitative loop-mediated isothermal amplification.

Han F, Wang F, Ge B.

Appl Environ Microbiol. 2011 Apr;77(8):2589-95. doi: 10.1128/AEM.02992-10. Epub 2011 Feb 25.

PMID:
21357428
[PubMed - indexed for MEDLINE]
Free PMC Article

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