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Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.

Tóth E, Weinhardt N, Bencsura P, Huszár K, Kulcsár PI, Tálas A, Fodor E, Welker E.

Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.


The epigenome: the next substrate for engineering.

Park M, Keung AJ, Khalil AS.

Genome Biol. 2016 Aug 31;17(1):183. doi: 10.1186/s13059-016-1046-5. Review.


Increasing the performance of pooled CRISPR-Cas9 drop-out screening.

Cross BC, Lawo S, Archer CR, Hunt JR, Yarker JL, Riccombeni A, Little AS, McCarthy NJ, Moore JD.

Sci Rep. 2016 Aug 22;6:31782. doi: 10.1038/srep31782.


High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize.

Qi W, Zhu T, Tian Z, Li C, Zhang W, Song R.

BMC Biotechnol. 2016 Aug 11;16(1):58. doi: 10.1186/s12896-016-0289-2.


Endogenous non-retroviral RNA virus elements evidence a novel type of antiviral immunity.

Honda T, Tomonaga K.

Mob Genet Elements. 2016 Mar 22;6(3):e1165785. doi: 10.1080/2159256X.2016.1165785. eCollection 2016 May-Jun.


A 'suicide' CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans.

Wang Y, Wei D, Zhu X, Pan J, Zhang P, Huo L, Zhu X.

Sci Rep. 2016 Aug 9;6:31145. doi: 10.1038/srep31145.


The CRISPR/Cas9 system for gene editing and its potential application in pain research.

Sun L, Lutz BM, Tao YX.

Transl Perioper Pain Med. 2016;1(3):22-33.


BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment.

Boel A, Steyaert W, De Rocker N, Menten B, Callewaert B, De Paepe A, Coucke P, Willaert A.

Sci Rep. 2016 Jul 27;6:30330. doi: 10.1038/srep30330.


Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer.

Liu G, Liu K, Wei H, Li L, Zhang S.

Mol Med Rep. 2016 Sep;14(3):2527-33. doi: 10.3892/mmr.2016.5530. Epub 2016 Jul 19.


Evolutionary Ecology of Prokaryotic Immune Mechanisms.

van Houte S, Buckling A, Westra ER.

Microbiol Mol Biol Rev. 2016 Jul 13;80(3):745-63. doi: 10.1128/MMBR.00011-16. Print 2016 Sep. Review.


A Broad Overview and Review of CRISPR-Cas Technology and Stem Cells.

Waddington SN, Privolizzi R, Karda R, O'Neill HC.

Curr Stem Cell Rep. 2016;2:9-20. Epub 2016 Feb 11. Review.


CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections.

van Diemen FR, Kruse EM, Hooykaas MJ, Bruggeling CE, Schürch AC, van Ham PM, Imhof SM, Nijhuis M, Wiertz EJ, Lebbink RJ.

PLoS Pathog. 2016 Jun 30;12(6):e1005701. doi: 10.1371/journal.ppat.1005701. eCollection 2016 Jun.


CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973.

Wendt KE, Ungerer J, Cobb RE, Zhao H, Pakrasi HB.

Microb Cell Fact. 2016 Jun 23;15(1):115. doi: 10.1186/s12934-016-0514-7.


Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency.

Zhang JP, Li XL, Neises A, Chen W, Hu LP, Ji GZ, Yu JY, Xu J, Yuan WP, Cheng T, Zhang XB.

Sci Rep. 2016 Jun 24;6:28566. doi: 10.1038/srep28566.


Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI.

Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP.

Dev Dyn. 2016 Sep;245(9):925-36. doi: 10.1002/dvdy.24426. Epub 2016 Jul 25.


The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers.

Luo ML, Jackson RN, Denny SR, Tokmina-Lukaszewska M, Maksimchuk KR, Lin W, Bothner B, Wiedenheft B, Beisel CL.

Nucleic Acids Res. 2016 Sep 6;44(15):7385-94. doi: 10.1093/nar/gkw421. Epub 2016 May 12.


Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes.

Oliveros JC, Franch M, Tabas-Madrid D, San-León D, Montoliu L, Cubas P, Pazos F.

Nucleic Acids Res. 2016 Jul 8;44(W1):W267-71. doi: 10.1093/nar/gkw407. Epub 2016 May 10.


Genome Editing of the CYP1A1 Locus in iPSCs as a Platform to Map AHR Expression throughout Human Development.

Smith BW, Stanford EA, Sherr DH, Murphy GJ.

Stem Cells Int. 2016;2016:2574152. doi: 10.1155/2016/2574152. Epub 2016 Apr 11.


In Vivo Delivery Systems for Therapeutic Genome Editing.

Wang L, Li F, Dang L, Liang C, Wang C, He B, Liu J, Li D, Wu X, Xu X, Lu A, Zhang G.

Int J Mol Sci. 2016 Apr 27;17(5). pii: E626. doi: 10.3390/ijms17050626. Review.

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