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Items: 1 to 20 of 36

1.

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels.

Basak S, Chatterjee S, Chakrapani S.

J Vis Exp. 2016 Jul 4;(113). doi: 10.3791/54127.

PMID:
27403967
2.

Peptide-Membrane Interactions by Spin-Labeling EPR.

Smirnova TI, Smirnov AI.

Methods Enzymol. 2015;564:219-58. doi: 10.1016/bs.mie.2015.08.018. Epub 2015 Sep 26.

3.

Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.

Le Breton N, Martinho M, Mileo E, Etienne E, Gerbaud G, Guigliarelli B, Belle V.

Front Mol Biosci. 2015 May 19;2:21. doi: 10.3389/fmolb.2015.00021. eCollection 2015. Review.

4.

EPR Studies of Gating Mechanisms in Ion Channels.

Chakrapani S.

Methods Enzymol. 2015;557:279-306. doi: 10.1016/bs.mie.2014.12.030. Epub 2015 Mar 24. Review.

5.

Structural studies of proteins by paramagnetic solid-state NMR spectroscopy.

Jaroniec CP.

J Magn Reson. 2015 Apr;253:50-9. doi: 10.1016/j.jmr.2014.12.017.

6.

Mapping the membrane topography of the TH6-TH7 segment of the diphtheria toxin T-domain channel.

Kienker PK, Wu Z, Finkelstein A.

J Gen Physiol. 2015 Feb;145(2):107-25. doi: 10.1085/jgp.201411326. Epub 2015 Jan 12.

7.

Mapping membrane protein backbone dynamics: a comparison of site-directed spin labeling with NMR 15N-relaxation measurements.

Lo RH, Kroncke BM, Solomon TL, Columbus L.

Biophys J. 2014 Oct 7;107(7):1697-702. doi: 10.1016/j.bpj.2014.08.018.

8.

Characterizing solution surface loop conformational flexibility of the GM2 activator protein.

Carter JD, Mathias JD, Gomez EF, Ran Y, Xu F, Galiano L, Tran NQ, D'Amore PW, Wright CS, Chakravorty DK, Fanucci GE.

J Phys Chem B. 2014 Sep 11;118(36):10607-17. doi: 10.1021/jp505938t. Epub 2014 Aug 26.

9.

Molecular interactions involved in proton-dependent gating in KcsA potassium channels.

Posson DJ, Thompson AN, McCoy JG, Nimigean CM.

J Gen Physiol. 2013 Dec;142(6):613-24. doi: 10.1085/jgp.201311057. Epub 2013 Nov 11.

10.

Conformational transitions underlying pore opening and desensitization in membrane-embedded Gloeobacter violaceus ligand-gated ion channel (GLIC).

Velisetty P, Chalamalasetti SV, Chakrapani S.

J Biol Chem. 2012 Oct 26;287(44):36864-72. doi: 10.1074/jbc.M112.401067. Epub 2012 Sep 13.

11.

Identification and removal of nitroxide spin label contaminant: impact on PRE studies of α-helical membrane proteins in detergent.

Kroncke BM, Columbus L.

Protein Sci. 2012 Apr;21(4):589-95. doi: 10.1002/pro.2038. Epub 2012 Mar 2.

12.

Opposite movement of the external gate of a glutamate transporter homolog upon binding cotransported sodium compared with substrate.

Focke PJ, Moenne-Loccoz P, Larsson HP.

J Neurosci. 2011 Apr 20;31(16):6255-62. doi: 10.1523/JNEUROSCI.6096-10.2011.

13.

A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity.

Isom DG, Marguet PR, Oas TG, Hellinga HW.

Proteins. 2011 Apr;79(4):1034-47. doi: 10.1002/prot.22932. Epub 2011 Jan 5.

14.

RosettaEPR: an integrated tool for protein structure determination from sparse EPR data.

Hirst SJ, Alexander N, McHaourab HS, Meiler J.

J Struct Biol. 2011 Mar;173(3):506-14. doi: 10.1016/j.jsb.2010.10.013. Epub 2010 Oct 26.

15.

Structural origins of nitroxide side chain dynamics on membrane protein α-helical sites.

Kroncke BM, Horanyi PS, Columbus L.

Biochemistry. 2010 Nov 30;49(47):10045-60. doi: 10.1021/bi101148w. Epub 2010 Nov 8.

16.

Three- and four-repeat Tau coassemble into heterogeneous filaments: an implication for Alzheimer disease.

Siddiqua A, Margittai M.

J Biol Chem. 2010 Nov 26;285(48):37920-6. doi: 10.1074/jbc.M110.185728. Epub 2010 Oct 4.

17.

Characterization of the PCMBS-dependent modification of KCa3.1 channel gating.

Bailey MA, Grabe M, Devor DC.

J Gen Physiol. 2010 Oct;136(4):367-87. doi: 10.1085/jgp.201010430. Epub 2010 Sep 13.

18.

Conformational changes in BAK, a pore-forming proapoptotic Bcl-2 family member, upon membrane insertion and direct evidence for the existence of BH3-BH3 contact interface in BAK homo-oligomers.

Oh KJ, Singh P, Lee K, Foss K, Lee S, Park M, Lee S, Aluvila S, Park M, Singh P, Kim RS, Symersky J, Walters DE.

J Biol Chem. 2010 Sep 10;285(37):28924-37. doi: 10.1074/jbc.M110.135293. Epub 2010 Jul 6.

19.

Electron spin-echo envelope modulation (ESEEM) reveals water and phosphate interactions with the KcsA potassium channel.

Cieslak JA, Focia PJ, Gross A.

Biochemistry. 2010 Feb 23;49(7):1486-94. doi: 10.1021/bi9016523.

20.

Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation.

Pavlova A, McCarney ER, Peterson DW, Dahlquist FW, Lew J, Han S.

Phys Chem Chem Phys. 2009 Aug 21;11(31):6833-9. doi: 10.1039/b906101k. Epub 2009 Jun 29.

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