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Items: 1 to 20 of 92

1.

Improved properties of FLP recombinase evolved by cycling mutagenesis.

Buchholz F, Angrand PO, Stewart AF.

Nat Biotechnol. 1998 Jul;16(7):657-62.

PMID:
9661200
2.

Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination.

Buchholz F, Ringrose L, Angrand PO, Rossi F, Stewart AF.

Nucleic Acids Res. 1996 Nov 1;24(21):4256-62.

3.
6.

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Datsenko KA, Wanner BL.

Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5.

7.

Alteration of Cre recombinase site specificity by substrate-linked protein evolution.

Buchholz F, Stewart AF.

Nat Biotechnol. 2001 Nov;19(11):1047-52.

PMID:
11689850
8.

The Flp double cross system a simple efficient procedure for cloning DNA fragments.

Sadowski PD.

BMC Biotechnol. 2003 Jul 18;3:9. Epub 2003 Jul 18.

9.

Chimeras of the Flp and Cre recombinases: tests of the mode of cleavage by Flp and Cre.

Shaikh AC, Sadowski PD.

J Mol Biol. 2000 Sep 8;302(1):27-48.

PMID:
10964559
10.

Engineering cell-permeant FLP recombinase for tightly controlled inducible and reversible overexpression in embryonic stem cells.

Patsch C, Peitz M, Otte DM, Kesseler D, Jungverdorben J, Wunderlich FT, Brüstle O, Zimmer A, Edenhofer F.

Stem Cells. 2010 May;28(5):894-902. doi: 10.1002/stem.417.

11.

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.

Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M.

Biochem Biophys Res Commun. 2002 May 10;293(3):953-7.

PMID:
12051751
12.

Cell-specific ecdysone-inducible expression of FLP recombinase in mammalian cells.

Sawicki JA, Monks B, Morris RJ.

Biotechniques. 1998 Nov;25(5):868-70, 872-5.

PMID:
9821589
13.

[Prokaryotic expression of DNA recombinase FLP and its purification with enzymatic activity].

Gai Y, Wang WQ, Lu H, Jiang XN.

Sheng Wu Gong Cheng Xue Bao. 2007 Jul;23(4):724-9. Chinese.

PMID:
17822052
14.

Structural plasticity of the Flp-Holliday junction complex.

Conway AB, Chen Y, Rice PA.

J Mol Biol. 2003 Feb 14;326(2):425-34.

PMID:
12559911
15.

New insights into host factor requirements for prokaryotic beta-recombinase-mediated reactions in mammalian cells.

Diaz V, Servert P, Prieto I, Gonzalez MA, Martinez-A C, Alonso JC, Bernad A.

J Biol Chem. 2001 May 11;276(19):16257-64. Epub 2001 Jan 30.

16.

Recombinase-mediated cassette exchange (RMCE) and BAC engineering via VCre/VloxP and SCre/SloxP systems.

Minorikawa S, Nakayama M.

Biotechniques. 2011 Apr;50(4):235-46. doi: 10.2144/000113649.

17.

Comparative kinetic analysis of FLP and cre recombinases: mathematical models for DNA binding and recombination.

Ringrose L, Lounnas V, Ehrlich L, Buchholz F, Wade R, Stewart AF.

J Mol Biol. 1998 Nov 27;284(2):363-84.

PMID:
9813124
18.

Efficient FLP recombination in mouse ES cells and oocytes.

Schaft J, Ashery-Padan R, van der Hoeven F, Gruss P, Stewart AF.

Genesis. 2001 Sep;31(1):6-10.

PMID:
11668672
19.

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates.

Voehringer D, Wu D, Liang HE, Locksley RM.

BMC Biotechnol. 2009 Jul 28;9:69. doi: 10.1186/1472-6750-9-69.

20.

Comparison of efficiency between FLPe and Cre for recombinase-mediated cassette exchange in vitro and in adenovirus vector production.

Takata Y, Kondo S, Goda N, Kanegae Y, Saito I.

Genes Cells. 2011 Jul;16(7):765-77. doi: 10.1111/j.1365-2443.2011.01526.x.

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