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33176883,36448813,36917471,35579554,31787070,28327976,35695508,27156482,29554948,36475839,33020656,32788623,31238873,33957869,31769805,36399059,30502941,36927511,32727443,32171248,29183281,33158461,34512586,29925315,31483244,30621760,33758906,28968737,36513983,30523036,30594129,34649602,32093654,31359005,28122610,30180802,34090540,30815668,31296857,36457010,33277629,30352611,30860572,35302439,34586399,31089679,34996356,30967123,31772173,25927464,33716184,31406327,29788930,34294039,20008478,34299288,33790294,26861018,24083400,34301928,35945546,30661755,33961222,34730411,30626323,24564377,24607570,22384016,36804804,27822530,26442255,23185243,26641475,24134808,36289209,26377912,28638050,30298255,35633679,34039264,31375809,29106455,34641955,27793084,31856721,33422152,33101371,35189932,26250558,34446072,36316646,27154001,26678408,27243603,28433722,33580146,27831497,29258419,33606255,35904764,35298370,32027927,33303873,25859745,35679948,25218180,29227468,30126356,26434730,33001022,21486487,28112173,29678199,35534630,33710295,33850654,32761733,31672156,24950923,31375138,28103917,27712583,25572018,20122183,26293485,26718401,36445112,33597514,33319909,19908352,30010758,31187446,24034426,36939007,22821567,36229545,34717540,27282101,22495746,27343682,35404122,31794015,25188499,35068060,29953874,28369353,32219339,35234490,34453168,29040406,32471482,36614092,35627213,23667655,32153100,35077539,32801147,24564472,35430490,35172890,33781324,36519933,23593174,36699587,33592536,35646097,35397610,36369227,30669388,36526983,37120601,33768248,22192763,35642524,25270300,36167684,34354114,32042169,35703559,36367700,35972375,34641782,35546658,34891160,34170814,35668290,30584534,18083777,33225533,34980911,32871010,34995394,34132589,35414589,24090403,36352504,30624602,22030673,19796657,32540955,35067170,35924489,25148512,35567771,33845884,34570223,36744896,33565054,35136954,22208765,37039735,23717627,24179701,20958248,34702846,35193521,35776122,24931998,36933058,20576136,33347571,22154471,32893860,31984131,32556169,31167634,36735822,23922726,36335499,34521459,26968756,36368923,34311761,34864203,25319552,32065492,23550143,34791313,36038049,34247248,35680095,33288906,36892439,30266101,32449778,37010293,36239393,28158639,31119088,34415535,32385271,32612216,35848773,36214570,33318652,32810209,36972309,34525345,33035301,22941634,34313980,36171387,34488624,36307411,28369246,35511110,19208115,34478378,32616517,35336091,33349699,33094089,23268604,24564333,35356467,33482026,33561126,20676378,32686750,36232783,35073327,32163141,20388197,33857229,32603623,29426289,36973656,25253095,36575570,24124490,24827833,35993719,35315560,23435317,23902516,24564274,36813568,32506266,34492339,33803228,36324140,37149537,25252700,35940845,20081835,20805240,36376589,18340039,35594250,34606462,33987534,37155831,35849350,36631912,23544073,21915294,35821155,24142950,36639368,20724458,34090340,35818005,33961223,36270681,34619757,22962446,23516532,37027361,36073806,21281493,35120119,20678236,32937018,34812649,35837476,25122209,34330336,22577288,35938717,37114640,24281688,36308393,21040513,32520351,33656283,33398153,35484115,35037209,25159659,35525918,35648784,31848574,36929080,20962877,34232887,34900140,34775997,35361931,36838385,37149708,31168775,32558637,35939836,35396482,20877643,33961733,36635540,33598708,22747657,34410641,35171093,24837716,37142439,34819031,35561209,37000853,25099007,21948828,32883749,35727019,35946988,34078257,34508117,33419473,20019144,36453992,34869767,34949778,36335494,25252785,35227283,34671046,25253180,35676264,35417481,20831800,36065055,33996146,23251337,23644548,32859925,19087275,33727564,24846174,22347999,31891535,35746732,23663384,32577746,36869788,32382098,36508259,21651990,35756038,24437399,22847406,36209213,36824328,35729161,23259615,32657355,22174807,23029438,33765910,35789207,20508146,25329997,37066122,35259160,36604505,33864056,19056694,35248155,23082188,36792366,36650186,24094745,22574660,32687865,31711268,22247176,33922793,36212901,22846331,33530937,35710651,24855317,35784064,29941292,33910595,35761554,24418700,35672336,36712019,34172093,34264958,35216302,36781718,20123915,23216677,34370725,25294837,35546409,36618978,23481336,32188701,35802409,32504078,33785903,29767702,32813091,24443382,23734710,36050551,35765182,34868218,35279076,33349238,22792313,21408061,24069303,26883533,22162995,37085924,20634954,23202435,33148306,34051323,36469554,35962900,34410642,24053649,36057561,35118380,24353264,25252999,34152413,34249520,21546400,34603381,34706052,19909532,19542148,32864214,35640223,26286554,34795375,33961626,34202675,36151788,33726674,24454771,30320765,36657438,25210663,35336193,34184911,32523024,23550823,35067223,22132069,36335985,34301627,34991471,34654941,35695379,35247565,34040632,32926900,36910196,34484261,32998695,33868775,32589667,36282811,36763048,33434206,36178955,22039460,35332338,36557669,34814051,35136576,35563041,19287394,36706059,33789731,23047562,32434845,31788019,24297520,35999507,34078284,20064276,24886150,35533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A comprehensive investigation of metagenome assembly by linked-read sequencing.

Zhang L, Fang X, Liao H, Zhang Z, Zhou X, Han L, Chen Y, Qiu Q, Li SC. Zhang L, et al. Microbiome. 2020 Nov 11;8(1):156. doi: 10.1186/s40168-020-00929-3. Microbiome. 2020. PMID: 33176883 Free PMC article.

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes.

Patin NV, Goodwin KD. Patin NV, et al. mSystems. 2022 Dec 20;7(6):e0059522. doi: 10.1128/msystems.00595-22. Epub 2022 Nov 30. mSystems. 2022. PMID: 36448813 Free PMC article.

Benchmarking genome assembly methods on metagenomic sequencing data.

Zhang Z, Yang C, Veldsman WP, Fang X, Zhang L. Zhang Z, et al. Brief Bioinform. 2023 Mar 19;24(2):bbad087. doi: 10.1093/bib/bbad087. Brief Bioinform. 2023. PMID: 36917471

Generation and application of pseudo-long reads for metagenome assembly.

Sim M, Lee J, Wy S, Park N, Lee D, Kwon D, Kim J. Sim M, et al. Gigascience. 2022 May 17;11:giac044. doi: 10.1093/gigascience/giac044. Gigascience. 2022. PMID: 35579554 Free PMC article.

Intestinal microbiota domination under extreme selective pressures characterized by metagenomic read cloud sequencing and assembly.

Kang JB, Siranosian BA, Moss EL, Banaei N, Andermann TM, Bhatt AS. Kang JB, et al. BMC Bioinformatics. 2019 Dec 2;20(Suppl 16):585. doi: 10.1186/s12859-019-3073-1. BMC Bioinformatics. 2019. PMID: 31787070 Free PMC article.

MinION™ nanopore sequencing of environmental metagenomes: a synthetic approach.

Brown BL, Watson M, Minot SS, Rivera MC, Franklin RB. Brown BL, et al. Gigascience. 2017 Mar 1;6(3):1-10. doi: 10.1093/gigascience/gix007. Gigascience. 2017. PMID: 28327976 Free PMC article.

Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples.

Zaragoza-Solas A, Haro-Moreno JM, Rodriguez-Valera F, López-Pérez M. Zaragoza-Solas A, et al. mSystems. 2022 Jun 28;7(3):e0019222. doi: 10.1128/msystems.00192-22. Epub 2022 Jun 13. mSystems. 2022. PMID: 35695508 Free PMC article.

Improved metagenome assemblies and taxonomic binning using long-read circular consensus sequence data.

Frank JA, Pan Y, Tooming-Klunderud A, Eijsink VGH, McHardy AC, Nederbragt AJ, Pope PB. Frank JA, et al. Sci Rep. 2016 May 9;6:25373. doi: 10.1038/srep25373. Sci Rep. 2016. PMID: 27156482 Free PMC article.

Species classifier choice is a key consideration when analysing low-complexity food microbiome data.

Walsh AM, Crispie F, O'Sullivan O, Finnegan L, Claesson MJ, Cotter PD. Walsh AM, et al. Microbiome. 2018 Mar 20;6(1):50. doi: 10.1186/s40168-018-0437-0. Microbiome. 2018. PMID: 29554948 Free PMC article.

Improved Assembly of Metagenome-Assembled Genomes and Viruses in Tibetan Saline Lake Sediment by HiFi Metagenomic Sequencing.

Tao Y, Xun F, Zhao C, Mao Z, Li B, Xing P, Wu QL. Tao Y, et al. Microbiol Spectr. 2023 Feb 14;11(1):e0332822. doi: 10.1128/spectrum.03328-22. Epub 2022 Dec 8. Microbiol Spectr. 2023. PMID: 36475839 Free PMC article.

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