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Results: 1 to 20 of 96

1.

The workflow of single-cell expression profiling using quantitative real-time PCR.

Ståhlberg A, Kubista M.

Expert Rev Mol Diagn. 2014 Apr;14(3):323-31. doi: 10.1586/14737159.2014.901154.

PMID:
24649819
[PubMed - in process]
2.

[Use of the real-time RT-PCR method for investigation of small stable RNA expression level in human epidermoid carcinoma cells A431].

Nikitina TV, Nazarova NIu, Tishchenko LI, Tuohimaa P, Sedova VM.

Tsitologiia. 2003;45(4):392-402. Russian.

PMID:
14520871
[PubMed - indexed for MEDLINE]
3.

Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays.

Jang JS, Simon VA, Feddersen RM, Rakhshan F, Schultz DA, Zschunke MA, Lingle WL, Kolbert CP, Jen J.

BMC Genomics. 2011 Mar 9;12:144. doi: 10.1186/1471-2164-12-144.

PMID:
21388556
[PubMed - indexed for MEDLINE]
Free PMC Article
4.

Profiling of pre-micro RNAs and microRNAs using quantitative real-time PCR (qPCR) arrays.

Chugh P, Tamburro K, Dittmer DP.

J Vis Exp. 2010 Dec 3;(46). pii: 2210. doi: 10.3791/2210.

PMID:
21178961
[PubMed - indexed for MEDLINE]
Free PMC Article
5.

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA.

Mestdagh P, Feys T, Bernard N, Guenther S, Chen C, Speleman F, Vandesompele J.

Nucleic Acids Res. 2008 Dec;36(21):e143. doi: 10.1093/nar/gkn725. Epub 2008 Oct 21.

PMID:
18940866
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

RT-qPCR work-flow for single-cell data analysis.

Ståhlberg A, Rusnakova V, Forootan A, Anderova M, Kubista M.

Methods. 2013 Jan;59(1):80-8. doi: 10.1016/j.ymeth.2012.09.007. Epub 2012 Sep 25.

PMID:
23021995
[PubMed - indexed for MEDLINE]
7.

Gene expression analysis in biomarker research and early drug development using function tested reverse transcription quantitative real-time PCR assays.

Lohmann S, Herold A, Bergauer T, Belousov A, Betzl G, Demario M, Dietrich M, Luistro L, Poignée-Heger M, Schostack K, Simcox M, Walch H, Yin X, Zhong H, Weisser M.

Methods. 2013 Jan;59(1):10-9. doi: 10.1016/j.ymeth.2012.07.003. Epub 2012 Jul 14.

PMID:
22796720
[PubMed - indexed for MEDLINE]
Free Article
8.

Improved quantitative real-time RT-PCR for expression profiling of individual cells.

Liss B.

Nucleic Acids Res. 2002 Sep 1;30(17):e89.

PMID:
12202777
[PubMed - indexed for MEDLINE]
Free PMC Article
9.

Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis.

Mohammadi-Yeganeh S, Paryan M, Mirab Samiee S, Soleimani M, Arefian E, Azadmanesh K, Mostafavi E, Mahdian R, Karimipoor M.

Mol Biol Rep. 2013 May;40(5):3665-74. doi: 10.1007/s11033-012-2442-x. Epub 2013 Jan 10.

PMID:
23307300
[PubMed - indexed for MEDLINE]
10.

Quantification noise in single cell experiments.

Reiter M, Kirchner B, Müller H, Holzhauer C, Mann W, Pfaffl MW.

Nucleic Acids Res. 2011 Oct;39(18):e124. doi: 10.1093/nar/gkr505. Epub 2011 Jul 11. Erratum in: Nucleic Acids Res. 2011 Dec;39(22):9834.

PMID:
21745823
[PubMed - indexed for MEDLINE]
Free PMC Article
11.

CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.

Boeuf P, Vigan-Womas I, Jublot D, Loizon S, Barale JC, Akanmori BD, Mercereau-Puijalon O, Behr C.

BMC Immunol. 2005 Mar 4;6:5.

PMID:
15748278
[PubMed - indexed for MEDLINE]
Free PMC Article
12.

How to do successful gene expression analysis using real-time PCR.

Derveaux S, Vandesompele J, Hellemans J.

Methods. 2010 Apr;50(4):227-30. doi: 10.1016/j.ymeth.2009.11.001. Epub 2009 Dec 5.

PMID:
19969088
[PubMed - indexed for MEDLINE]
13.

Twenty-five years of quantitative PCR for gene expression analysis.

VanGuilder HD, Vrana KE, Freeman WM.

Biotechniques. 2008 Apr;44(5):619-26. doi: 10.2144/000112776. Review.

PMID:
18474036
[PubMed - indexed for MEDLINE]
Free Article
14.

Global expression profiling of rice microRNAs by one-tube stem-loop reverse transcription quantitative PCR revealed important roles of microRNAs in abiotic stress responses.

Shen J, Xie K, Xiong L.

Mol Genet Genomics. 2010 Dec;284(6):477-88. doi: 10.1007/s00438-010-0581-0. Epub 2010 Oct 13.

PMID:
20941508
[PubMed - indexed for MEDLINE]
15.

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.

Bengtsson M, Hemberg M, Rorsman P, Ståhlberg A.

BMC Mol Biol. 2008 Jul 17;9:63. doi: 10.1186/1471-2199-9-63.

PMID:
18631407
[PubMed - indexed for MEDLINE]
Free PMC Article
16.

A novel qPCR assay for viral encoded microRNAs.

Reichenstein I, Aizenberg N, Goshen M, Bentwich Z, Avni YS.

J Virol Methods. 2010 Feb;163(2):323-8. doi: 10.1016/j.jviromet.2009.10.018. Epub 2009 Oct 29.

PMID:
19879298
[PubMed - indexed for MEDLINE]
17.

Quantification of chemotherapeutic target gene mRNA expression in human breast cancer biopsies: comparison of real-time reverse transcription-PCR vs. relative quantification reverse transcription-PCR utilizing DNA sequencer analysis of PCR products.

Juhasz A, Frankel P, Cheng C, Rivera H, Vishwanath R, Chiu A, Margolin K, Yen Y, Newman EM, Synold T, Wilczynski S, Lenz HJ, Gandara D, Albain KS, Longmate J, Doroshow JH.

J Clin Lab Anal. 2003;17(5):184-94.

PMID:
12938148
[PubMed - indexed for MEDLINE]
18.

miR-Q: a novel quantitative RT-PCR approach for the expression profiling of small RNA molecules such as miRNAs in a complex sample.

Sharbati-Tehrani S, Kutz-Lohroff B, Bergbauer R, Scholven J, Einspanier R.

BMC Mol Biol. 2008 Apr 10;9:34. doi: 10.1186/1471-2199-9-34.

PMID:
18400113
[PubMed - indexed for MEDLINE]
Free PMC Article
19.

Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis.

Chen Y, Gelfond JA, McManus LM, Shireman PK.

BMC Genomics. 2009 Aug 28;10:407. doi: 10.1186/1471-2164-10-407.

PMID:
19715577
[PubMed - indexed for MEDLINE]
Free PMC Article
20.

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs.

Varkonyi-Gasic E, Wu R, Wood M, Walton EF, Hellens RP.

Plant Methods. 2007 Oct 12;3:12.

PMID:
17931426
[PubMed]
Free PMC Article

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