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Results: 1 to 20 of 102

1.

A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107.

Zhang L, Tang X, Cui D, Yao Z, Gao B, Jiang S, Yin B, Yuan YA, Wei D.

Protein Sci. 2014 Jan;23(1):110-6. doi: 10.1002/pro.2388. Epub 2013 Nov 22.

PMID:
24353171
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

Structure, mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way.

Zhang L, Gao B, Yuan Z, He X, Yuan YA, Zhang JZ, Wei D.

Biochim Biophys Acta. 2014 Jul;1844(7):1183-92. doi: 10.1016/j.bbapap.2014.02.021. Epub 2014 Mar 4.

PMID:
24602769
[PubMed - indexed for MEDLINE]
3.

Template-based modeling of a psychrophilic lipase: conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols.

Xu T, Gao B, Zhang L, Lin J, Wang X, Wei D.

Biochim Biophys Acta. 2010 Dec;1804(12):2183-90. doi: 10.1016/j.bbapap.2010.08.012. Epub 2010 Sep 7.

PMID:
20828637
[PubMed - indexed for MEDLINE]
4.

Zinc in lipase L1 from Geobacillus stearothermophilus L1 and structural implications on thermal stability.

Choi WC, Kim MH, Ro HS, Ryu SR, Oh TK, Lee JK.

FEBS Lett. 2005 Jun 20;579(16):3461-6.

PMID:
15949807
[PubMed - indexed for MEDLINE]
Free Article
5.

Enhanced enzyme kinetic stability by increasing rigidity within the active site.

Xie Y, An J, Yang G, Wu G, Zhang Y, Cui L, Feng Y.

J Biol Chem. 2014 Mar 14;289(11):7994-8006. doi: 10.1074/jbc.M113.536045. Epub 2014 Jan 21.

PMID:
24448805
[PubMed - indexed for MEDLINE]
6.

Hydrophobic substitution of surface residues affects lipase stability in organic solvents.

Monsef Shokri M, Ahmadian S, Akbari N, Khajeh K.

Mol Biotechnol. 2014 Apr;56(4):360-8. doi: 10.1007/s12033-013-9716-y.

PMID:
24146432
[PubMed - indexed for MEDLINE]
7.

Engineering lipase A from mesophilic Bacillus subtilis for activity at low temperatures.

Kumar V, Yedavalli P, Gupta V, Rao NM.

Protein Eng Des Sel. 2014 Mar;27(3):73-82. doi: 10.1093/protein/gzt064. Epub 2014 Jan 8.

PMID:
24402332
[PubMed - indexed for MEDLINE]
8.

Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production.

Gao B, Su E, Lin J, Jiang Z, Ma Y, Wei D.

J Biotechnol. 2009 Jan 15;139(2):169-75. doi: 10.1016/j.jbiotec.2008.10.004. Epub 2008 Oct 22.

PMID:
19007827
[PubMed - indexed for MEDLINE]
9.

Protein engineering by random mutagenesis and structure-guided consensus of Geobacillus stearothermophilus Lipase T6 for enhanced stability in methanol.

Dror A, Shemesh E, Dayan N, Fishman A.

Appl Environ Microbiol. 2014 Feb;80(4):1515-27. doi: 10.1128/AEM.03371-13. Epub 2013 Dec 20.

PMID:
24362426
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica.

Wen S, Tan T, Zhao H.

J Biotechnol. 2012 Dec 15;164(2):248-53. doi: 10.1016/j.jbiotec.2012.08.023. Epub 2012 Sep 7.

PMID:
22982168
[PubMed - indexed for MEDLINE]
11.

Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase.

Gatti-Lafranconi P, Caldarazzo SM, Villa A, Alberghina L, Lotti M.

FEBS Lett. 2008 Jun 25;582(15):2313-8. doi: 10.1016/j.febslet.2008.05.037. Epub 2008 Jun 4.

PMID:
18534193
[PubMed - indexed for MEDLINE]
Free Article
12.

Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution.

Zhang N, Suen WC, Windsor W, Xiao L, Madison V, Zaks A.

Protein Eng. 2003 Aug;16(8):599-605.

PMID:
12968077
[PubMed - indexed for MEDLINE]
Free Article
13.

Cumulative site-directed charge-change replacements in bacteriophage T4 lysozyme suggest that long-range electrostatic interactions contribute little to protein stability.

Dao-pin S, Söderlind E, Baase WA, Wozniak JA, Sauer U, Matthews BW.

J Mol Biol. 1991 Oct 5;221(3):873-87.

PMID:
1942034
[PubMed - indexed for MEDLINE]
14.

Kinetic studies of Gly28:Ser mutant form of Bacillus pumilus lipase: changes in k(cat) and thermal dependence.

Bustos-Jaimes I, Mora-Lugo R, Calcagno ML, Farrés A.

Biochim Biophys Acta. 2010 Dec;1804(12):2222-7. doi: 10.1016/j.bbapap.2010.09.001. Epub 2010 Sep 8.

PMID:
20831908
[PubMed - indexed for MEDLINE]
15.

Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention.

Augustyniak W, Brzezinska AA, Pijning T, Wienk H, Boelens R, Dijkstra BW, Reetz MT.

Protein Sci. 2012 Apr;21(4):487-97. doi: 10.1002/pro.2031. Epub 2012 Feb 29.

PMID:
22267088
[PubMed - indexed for MEDLINE]
Free PMC Article
16.

Impact of the tryptophan residues of Humicola lanuginosa lipase on its thermal stability.

Zhu K, Jutila A, Tuominen EK, Patkar SA, Svendsen A, Kinnunen PK.

Biochim Biophys Acta. 2001 Jun 11;1547(2):329-38.

PMID:
11410289
[PubMed - indexed for MEDLINE]
17.

C-terminal domain of human pancreatic lipase is required for stability and maximal activity but not colipase reactivation.

Jennens ML, Lowe ME.

J Lipid Res. 1995 May;36(5):1029-36.

PMID:
7658150
[PubMed - indexed for MEDLINE]
Free Article
18.

Biochemical and structural characterization of two site-directed mutants of Staphylococcus xylosus lipase.

Kolling DJ, Bertoldo JB, Brod FC, Vernal J, Terenzi H, Arisi AC.

Mol Biotechnol. 2010 Oct;46(2):168-75. doi: 10.1007/s12033-010-9282-5.

PMID:
20387014
[PubMed - indexed for MEDLINE]
19.

Characterization and a point mutational approach of a psychrophilic lipase from an arctic bacterium, Bacillus pumilus.

Wi AR, Jeon SJ, Kim S, Park HJ, Kim D, Han SJ, Yim JH, Kim HW.

Biotechnol Lett. 2014 Jun;36(6):1295-302. doi: 10.1007/s10529-014-1475-8. Epub 2014 Feb 22.

PMID:
24563306
[PubMed - indexed for MEDLINE]
20.

Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface.

Tanaka S, Igarashi S, Ferri S, Sode K.

BMC Biochem. 2005 Feb 16;6:1.

PMID:
15715904
[PubMed - indexed for MEDLINE]
Free PMC Article

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