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The critical role of DNA extraction for detection of mycobacteria in tissues.

Radomski N, Kreitmann L, McIntosh F, Behr MA.

PLoS One. 2013 Oct 23;8(10):e78749. doi: 10.1371/journal.pone.0078749. eCollection 2013.


Comparison of fecal DNA extraction kits for the detection of Mycobacterium avium subsp. paratuberculosis by polymerase chain reaction.

Leite FL, Stokes KD, Robbe-Austerman S, Stabel JR.

J Vet Diagn Invest. 2013 Jan;25(1):27-34. doi: 10.1177/1040638712466395. Epub 2012 Nov 19.


New triplex real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces.

Schönenbrücher H, Abdulmawjood A, Failing K, Bülte M.

Appl Environ Microbiol. 2008 May;74(9):2751-8. doi: 10.1128/AEM.02534-07. Epub 2008 Mar 7.


Development of a novel DNA extraction method for identification and quantification of Mycobacterium avium subsp. paratuberculosis from tissue samples by real-time PCR.

Park KT, Allen AJ, Davis WC.

J Microbiol Methods. 2014 Apr;99:58-65. doi: 10.1016/j.mimet.2014.02.003. Epub 2014 Feb 15.


Use of the real-time PCR assay in conjunction with MagNA Pure for the detection of mycobacterial DNA from fixed specimens.

Beqaj SH, Flesher R, Walker GR, Smith SA.

Diagn Mol Pathol. 2007 Sep;16(3):169-73.


Evaluation of DNA extraction techniques for detecting Mycobacterium tuberculosis complex organisms in Asian elephant trunk wash samples.

Kay MK, Linke L, Triantis J, Salman MD, Larsen RS.

J Clin Microbiol. 2011 Feb;49(2):618-23. doi: 10.1128/JCM.00807-10. Epub 2010 Dec 15.


Pathogen quantitation in complex matrices: a multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition.

Pontiroli A, Travis ER, Sweeney FP, Porter D, Gaze WH, Mason S, Hibberd V, Holden J, Courtenay O, Wellington EM.

PLoS One. 2011 Mar 23;6(3):e17916. doi: 10.1371/journal.pone.0017916.


Efficient method for mycobacterial DNA extraction in blood cultures aids rapid PCR identification of Mycobacterium tuberculosis and Mycobacterium avium.

Nakatani SM, Burger M, Assef MC, Brockelt SR, Cogo LL, Messias-Reason IJ.

Eur J Clin Microbiol Infect Dis. 2004 Nov;23(11):851-4. Epub 2004 Oct 22.


Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium.

Amaro A, Duarte E, Amado A, Ferronha H, Botelho A.

Lett Appl Microbiol. 2008 Jul;47(1):8-11. doi: 10.1111/j.1472-765X.2008.02372.x. Epub 2008 May 21.


High-throughput direct fecal PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in sheep and cattle.

Plain KM, Marsh IB, Waldron AM, Galea F, Whittington AM, Saunders VF, Begg DJ, de Silva K, Purdie AC, Whittington RJ.

J Clin Microbiol. 2014 Mar;52(3):745-57. doi: 10.1128/JCM.03233-13. Epub 2013 Dec 18.


An inter-laboratory ring trial for the detection and isolation of Mycobacterium avium subsp. paratuberculosis from raw milk artificially contaminated with naturally infected faeces.

Donaghy JA, Rowe MT, Rademaker JL, Hammer P, Herman L, De Jonghe V, Blanchard B, Duhem K, Vindel E.

Food Microbiol. 2008 Feb;25(1):128-35. Epub 2007 Jul 14.


Evaluation of in situ methods used to detect Mycobacterium avium subsp. paratuberculosis in samples from patients with Crohn's disease.

Jeyanathan M, Alexander DC, Turenne CY, Girard C, Behr MA.

J Clin Microbiol. 2006 Aug;44(8):2942-50.


Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR.

Taylor GM, Worth DR, Palmer S, Jahans K, Hewinson RG.

BMC Vet Res. 2007 Jun 13;3:12.

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