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Items: 1 to 20 of 131

1.

Enhanced production of recombinant secretory proteins in Pichia pastoris by optimizing Kex2 P1' site.

Yang S, Kuang Y, Li H, Liu Y, Hui X, Li P, Jiang Z, Zhou Y, Wang Y, Xu A, Li S, Liu P, Wu D.

PLoS One. 2013 Sep 19;8(9):e75347. doi: 10.1371/journal.pone.0075347. eCollection 2013.

2.

Heterologous production of fungal effectors in Pichia pastoris.

Kombrink A.

Methods Mol Biol. 2012;835:209-17. doi: 10.1007/978-1-61779-501-5_13.

PMID:
22183656
3.
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Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence.

Eilert E, Rolf T, Heumaier A, Hollenberg CP, Piontek M, Suckow M.

J Biotechnol. 2013 Aug 20;167(2):94-100. doi: 10.1016/j.jbiotec.2012.08.024. Epub 2012 Sep 7.

PMID:
22982399
5.

A new signal sequence for recombinant protein secretion in Pichia pastoris.

Govindappa N, Hanumanthappa M, Venkatarangaiah K, Periyasamy S, Sreenivas S, Soni R, Sastry K.

J Microbiol Biotechnol. 2014 Mar 28;24(3):337-45.

6.

Overview of protein expression in Pichia pastoris.

Higgins DR.

Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.7. doi: 10.1002/0471140864.ps0507s02.

PMID:
18429188
7.

High-yield secretion of recombinant gelatins by Pichia pastoris.

Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, de Wolf FA.

Yeast. 1999 Aug;15(11):1087-96.

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10.

High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors.

Sasagawa T, Matsui M, Kobayashi Y, Otagiri M, Moriya S, Sakamoto Y, Ito Y, Lee CC, Kitamoto K, Arioka M.

Plasmid. 2011 Jan;65(1):65-9. doi: 10.1016/j.plasmid.2010.08.004. Epub 2010 Aug 31.

PMID:
20807550
11.

Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization.

Heiss S, Puxbaum V, Gruber C, Altmann F, Mattanovich D, Gasser B.

Microbiology. 2015 Jul;161(7):1356-68. doi: 10.1099/mic.0.000105. Epub 2015 May 1.

PMID:
25934645
12.

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris.

Liang S, Li C, Ye Y, Lin Y.

Biotechnol Lett. 2013 Jan;35(1):97-105. doi: 10.1007/s10529-012-1055-8. Epub 2012 Sep 25.

PMID:
23160737
13.

Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris.

Juge N, Andersen JS, Tull D, Roepstorff P, Svensson B.

Protein Expr Purif. 1996 Sep;8(2):204-14.

PMID:
8812863
14.

Endoproteolytic processing of recombinant proalbumin variants by the yeast Kex2 protease.

Ledgerwood EC, George PM, Peach RJ, Brennan SO.

Biochem J. 1995 May 15;308 ( Pt 1):321-5.

15.

Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris.

Weidner M, Taupp M, Hallam SJ.

J Vis Exp. 2010 Feb 25;(36). pii: 1862. doi: 10.3791/1862.

16.

Enhancement in production of recombinant two-chain Insulin Glargine by over-expression of Kex2 protease in Pichia pastoris.

Sreenivas S, Krishnaiah SM, Govindappa N, Basavaraju Y, Kanojia K, Mallikarjun N, Natarajan J, Chatterjee A, Sastry KN.

Appl Microbiol Biotechnol. 2015 Jan;99(1):327-36. doi: 10.1007/s00253-014-6052-5. Epub 2014 Sep 20.

PMID:
25239036
17.

Influence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris.

Athmaram TN, Saraswat S, Singh AK, Rao MK, Gopalan N, Suryanarayana VV, Rao PV.

Virus Genes. 2012 Dec;45(3):440-51. doi: 10.1007/s11262-012-0809-7. Epub 2012 Sep 2.

PMID:
22940846
18.

Protein secretion in Pichia pastoris and advances in protein production.

Damasceno LM, Huang CJ, Batt CA.

Appl Microbiol Biotechnol. 2012 Jan;93(1):31-9. doi: 10.1007/s00253-011-3654-z. Epub 2011 Nov 6. Review.

PMID:
22057543
19.

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.

Jordà J, Jouhten P, Cámara E, Maaheimo H, Albiol J, Ferrer P.

Microb Cell Fact. 2012 May 8;11:57. doi: 10.1186/1475-2859-11-57.

20.

The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing.

Germain D, Dumas F, Vernet T, Bourbonnais Y, Thomas DY, Boileau G.

FEBS Lett. 1992 Mar 16;299(3):283-6.

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