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Results: 1 to 20 of 100

1.

Engineering soluble tobacco etch virus protease accompanies the loss of stability.

Fang J, Chen L, Cheng B, Fan J.

Protein Expr Purif. 2013 Nov;92(1):29-35. doi: 10.1016/j.pep.2013.08.015. Epub 2013 Sep 4.

PMID:
24012464
[PubMed - indexed for MEDLINE]
2.

In vivo and in vitro characterization of TEV protease mutants.

Wei L, Cai X, Qi Z, Rong L, Cheng B, Fan J.

Protein Expr Purif. 2012 Jun;83(2):157-63. doi: 10.1016/j.pep.2012.03.011. Epub 2012 Mar 28.

PMID:
22484199
[PubMed - indexed for MEDLINE]
3.

Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations.

Wang Y, Zhu GF, Ren SY, Han YG, Luo Y, Du LF.

J Mol Model. 2013 Nov;19(11):4865-75. doi: 10.1007/s00894-013-1930-9. Epub 2013 Sep 17.

PMID:
24043540
[PubMed - indexed for MEDLINE]
4.

Enhancing the stability and solubility of TEV protease using in silico design.

Cabrita LD, Gilis D, Robertson AL, Dehouck Y, Rooman M, Bottomley SP.

Protein Sci. 2007 Nov;16(11):2360-7. Epub 2007 Sep 28.

PMID:
17905838
[PubMed - indexed for MEDLINE]
Free PMC Article
5.

Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system.

Williams DJ, Puhl HL 3rd, Ikeda SR.

PLoS One. 2009 Oct 15;4(10):e7474. doi: 10.1371/journal.pone.0007474.

PMID:
19830250
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

A combined approach to improving large-scale production of tobacco etch virus protease.

Blommel PG, Fox BG.

Protein Expr Purif. 2007 Sep;55(1):53-68. Epub 2007 Apr 25.

PMID:
17543538
[PubMed - indexed for MEDLINE]
Free PMC Article
7.

Expression and purification of soluble His(6)-tagged TEV protease.

Tropea JE, Cherry S, Waugh DS.

Methods Mol Biol. 2009;498:297-307. doi: 10.1007/978-1-59745-196-3_19.

PMID:
18988033
[PubMed - indexed for MEDLINE]
8.

Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants.

Asano Y, Dadashipour M, Yamazaki M, Doi N, Komeda H.

Protein Eng Des Sel. 2011 Aug;24(8):607-16. doi: 10.1093/protein/gzr030. Epub 2011 Jul 5.

PMID:
21729945
[PubMed - indexed for MEDLINE]
Free Article
9.

Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli.

Folwarczna J, Moravec T, Plchova H, Hoffmeisterova H, Cerovska N.

Protein Expr Purif. 2012 Sep;85(1):152-7. doi: 10.1016/j.pep.2012.07.008. Epub 2012 Jul 28.

PMID:
22850536
[PubMed - indexed for MEDLINE]
10.

Improved solubility of TEV protease by directed evolution.

van den Berg S, Löfdahl PA, Härd T, Berglund H.

J Biotechnol. 2006 Feb 10;121(3):291-8. Epub 2005 Sep 15.

PMID:
16150509
[PubMed - indexed for MEDLINE]
11.

Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli.

de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B.

BMC Biotechnol. 2007 Jun 12;7:32.

PMID:
17565681
[PubMed - indexed for MEDLINE]
Free PMC Article
12.

Hyper-acidic protein fusion partners improve solubility and assist correct folding of recombinant proteins expressed in Escherichia coli.

Zou Z, Cao L, Zhou P, Su Y, Sun Y, Li W.

J Biotechnol. 2008 Jul 31;135(4):333-9. doi: 10.1016/j.jbiotec.2008.05.007. Epub 2008 May 27.

PMID:
18599143
[PubMed - indexed for MEDLINE]
13.

A novel method to determine the topology of peroxisomal membrane proteins in vivo using the tobacco etch virus protease.

Faber KN, Kram AM, Ehrmann M, Veenhuis M.

J Biol Chem. 2001 Sep 28;276(39):36501-7. Epub 2001 Jul 6.

PMID:
11443138
[PubMed - indexed for MEDLINE]
Free Article
14.

Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli.

Kataeva I, Chang J, Xu H, Luan CH, Zhou J, Uversky VN, Lin D, Horanyi P, Liu ZJ, Ljungdahl LG, Rose J, Luo M, Wang BC.

J Proteome Res. 2005 Nov-Dec;4(6):1942-51.

PMID:
16335938
[PubMed - indexed for MEDLINE]
15.

Upstream strategies to minimize proteolytic degradation upon recombinant production in Escherichia coli.

Murby M, Uhlén M, Ståhl S.

Protein Expr Purif. 1996 Mar;7(2):129-36. Review.

PMID:
8812844
[PubMed - indexed for MEDLINE]
16.

Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins.

Miladi B, El Marjou A, Boeuf G, Bouallagui H, Dufour F, Di Martino P, Elm'selmi A.

J Biotechnol. 2012 Apr 15;158(3):97-103. doi: 10.1016/j.jbiotec.2012.01.010. Epub 2012 Jan 24.

PMID:
22300512
[PubMed - indexed for MEDLINE]
17.

Controlled intracellular processing of fusion proteins by TEV protease.

Kapust RB, Waugh DS.

Protein Expr Purif. 2000 Jul;19(2):312-8.

PMID:
10873547
[PubMed - indexed for MEDLINE]
18.

[Tobacco etch virus proteinase: crystal structure of the active enzyme and its inactive mutant].

Zhdanov AS, Phan J, Evdokimov AG, Tropea JE, Kapust RB, Li M, Wlodawer A, Waugh DS.

Bioorg Khim. 2003 Sep-Oct;29(5):457-60. Russian.

PMID:
14601399
[PubMed - indexed for MEDLINE]
19.

An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization.

Fang L, Jia KZ, Tang YL, Ma DY, Yu M, Hua ZC.

Protein Expr Purif. 2007 Jan;51(1):102-9. Epub 2006 Jul 12.

PMID:
16919473
[PubMed - indexed for MEDLINE]
20.

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.

Kapust RB, Tözsér J, Fox JD, Anderson DE, Cherry S, Copeland TD, Waugh DS.

Protein Eng. 2001 Dec;14(12):993-1000.

PMID:
11809930
[PubMed - indexed for MEDLINE]
Free Article

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