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Items: 1 to 20 of 245

1.

Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter.

Fan L, Kadura I, Krebs LE, Larson JL, Bowden DM, Frye CC.

J Biotechnol. 2013 Dec;168(4):652-8. doi: 10.1016/j.jbiotec.2013.08.021. Epub 2013 Aug 29.

PMID:
23994266
2.

Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells.

Fan L, Kadura I, Krebs LE, Hatfield CC, Shaw MM, Frye CC.

Biotechnol Bioeng. 2012 Apr;109(4):1007-15. doi: 10.1002/bit.24365. Epub 2011 Nov 21.

PMID:
22068567
3.

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment.

Rajendra Y, Hougland MD, Alam R, Morehead TA, Barnard GC.

Biotechnol Bioeng. 2015 May;112(5):977-86. doi: 10.1002/bit.25514. Epub 2015 Mar 16.

PMID:
25502369
4.
5.

Development of transfection and high-producer screening protocols for the CHOK1SV cell system.

de la Cruz Edmonds MC, Tellers M, Chan C, Salmon P, Robinson DK, Markusen J.

Mol Biotechnol. 2006 Oct;34(2):179-90.

PMID:
17172663
6.
7.

A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression.

Daramola O, Stevenson J, Dean G, Hatton D, Pettman G, Holmes W, Field R.

Biotechnol Prog. 2014 Jan-Feb;30(1):132-41. doi: 10.1002/btpr.1809. Epub 2013 Oct 10.

PMID:
24106171
8.
9.

Early prediction of instability of Chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins.

Dorai H, Corisdeo S, Ellis D, Kinney C, Chomo M, Hawley-Nelson P, Moore G, Betenbaugh MJ, Ganguly S.

Biotechnol Bioeng. 2012 Apr;109(4):1016-30. doi: 10.1002/bit.24367. Epub 2011 Dec 1.

PMID:
22068683
10.

Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system.

Nakamura T, Omasa T.

J Biosci Bioeng. 2015 Sep;120(3):323-9. doi: 10.1016/j.jbiosc.2015.01.002. Epub 2015 Mar 17.

PMID:
25792187
11.

Auditioning of CHO host cell lines using the artificial chromosome expression (ACE) technology.

Kennard ML, Goosney DL, Monteith D, Roe S, Fischer D, Mott J.

Biotechnol Bioeng. 2009 Oct 15;104(3):526-39. doi: 10.1002/bit.22407.

PMID:
19544304
12.

[Construction of an anti-apoptosis CHO cell line for biopharmaceutical production].

Lai DZ, Fu L, Yu CM, Qi LQ, Weng SJ, Yu T, Wang HT, Chen W.

Sheng Wu Gong Cheng Xue Bao. 2003 May;19(3):322-6. Chinese.

PMID:
15969015
13.

A streamlined implementation of the glutamine synthetase-based protein expression system.

Knox R, Nettleship JE, Chang VT, Hui ZK, Santos AM, Rahman N, Ho LP, Owens RJ, Davis SJ.

BMC Biotechnol. 2013 Sep 24;13:74. doi: 10.1186/1472-6750-13-74.

14.

High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker.

Bebbington CR, Renner G, Thomson S, King D, Abrams D, Yarranton GT.

Biotechnology (N Y). 1992 Feb;10(2):169-75.

PMID:
1369477
15.

Amplification using CHO cell expression vectors.

Kingston RE, Kaufman RJ, Bebbington CR, Rolfe MR.

Curr Protoc Mol Biol. 2002 Nov;Chapter 16:Unit 16.23. doi: 10.1002/0471142727.mb1623s60.

PMID:
18265304
16.
17.

Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells.

Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG.

BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.

18.

Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.

Zhou H, Liu ZG, Sun ZW, Huang Y, Yu WY.

J Biotechnol. 2010 May 17;147(2):122-9. doi: 10.1016/j.jbiotec.2010.03.020. Epub 2010 Apr 3.

PMID:
20371256
19.

Effects of glutamine and asparagine on recombinant antibody production using CHO-GS cell lines.

Xu P, Dai XP, Graf E, Martel R, Russell R.

Biotechnol Prog. 2014 Nov-Dec;30(6):1457-68. doi: 10.1002/btpr.1957. Epub 2014 Aug 8.

PMID:
25079388
20.

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC, Derouazi M, Hacker DL, Beckmann JS, Wurm FM.

Biotechnol Bioeng. 2008 Dec 1;101(5):937-45. doi: 10.1002/bit.21972.

PMID:
18781700
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