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Items: 1 to 20 of 162

1.

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.

Jäger V, Büssow K, Wagner A, Weber S, Hust M, Frenzel A, Schirrmann T.

BMC Biotechnol. 2013 Jun 26;13:52. doi: 10.1186/1472-6750-13-52.

2.

A mammalian expression system for high throughput antibody screening.

Xu L, Jin X, Rainey GJ, Wu H, Gao C.

J Immunol Methods. 2013 Sep 30;395(1-2):45-53. doi: 10.1016/j.jim.2013.06.009. Epub 2013 Jul 4.

PMID:
23831609
3.

Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae.

Jørgensen ML, Friis NA, Just J, Madsen P, Petersen SV, Kristensen P.

Microb Cell Fact. 2014 Jan 15;13:9. doi: 10.1186/1475-2859-13-9.

4.

Production in yeast of pseudotype virus-like particles harboring functionally active antibody fragments neutralizing the cytolytic activity of vaginolysin.

Pleckaityte M, Zvirbliene A, Sezaite I, Gedvilaite A.

Microb Cell Fact. 2011 Dec 15;10:109. doi: 10.1186/1475-2859-10-109.

5.
6.

Expression of single-chain Fv-Fc fusions in Pichia pastoris.

Powers DB, Amersdorfer P, Poul M, Nielsen UB, Shalaby MR, Adams GP, Weiner LM, Marks JD.

J Immunol Methods. 2001 May 1;251(1-2):123-35.

PMID:
11292488
7.

Expression, purification and characterization of a human single-chain Fv antibody fragment fused with the Fc of an IgG1 targeting a rabies antigen in Pichia pastoris.

Wang DD, Su MM, Sun Y, Huang SL, Wang J, Yan WQ.

Protein Expr Purif. 2012 Nov;86(1):75-81. doi: 10.1016/j.pep.2012.08.015. Epub 2012 Sep 11.

PMID:
22982755
8.

A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format.

Xiao X, Chen Y, Mugabe S, Gao C, Tkaczyk C, Mazor Y, Pavlik P, Wu H, Dall'Acqua W, Chowdhury PS.

PLoS One. 2015 Oct 15;10(10):e0140691. doi: 10.1371/journal.pone.0140691. eCollection 2015.

9.

A simple vector system to improve performance and utilisation of recombinant antibodies.

Martin CD, Rojas G, Mitchell JN, Vincent KJ, Wu J, McCafferty J, Schofield DJ.

BMC Biotechnol. 2006 Dec 7;6:46.

10.

Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification.

Bujak E, Matasci M, Neri D, Wulhfard S.

Methods Mol Biol. 2014;1131:315-34. doi: 10.1007/978-1-62703-992-5_20.

PMID:
24515475
11.

Affinity maturation of antibodies: optimized methods to generate high-quality ScFv libraries and isolate IgG candidates by high-throughput screening.

Renaut L, Monnet C, Dubreuil O, Zaki O, Crozet F, Bouayadi K, Kharrat H, Mondon P.

Methods Mol Biol. 2012;907:451-61. doi: 10.1007/978-1-61779-974-7_26.

PMID:
22907368
12.

The influence of antibody fragment format on phage display based affinity maturation of IgG.

Steinwand M, Droste P, Frenzel A, Hust M, Dübel S, Schirrmann T.

MAbs. 2014 Jan-Feb;6(1):204-18.

13.

Baculovirus expression cassette vectors for rapid production of complete human IgG from phage display selected antibody fragments.

Liang M, Dübel S, Li D, Queitsch I, Li W, Bautz EK.

J Immunol Methods. 2001 Jan 1;247(1-2):119-30.

PMID:
11150543
14.

pSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins.

Griep RA, van Twisk C, Kerschbaumer RJ, Harper K, Torrance L, Himmler G, van der Wolf JM, Schots A.

Protein Expr Purif. 1999 Jun;16(1):63-9.

PMID:
10336861
15.
16.

A multi-Fc-species system for recombinant antibody production.

Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dübel S, Perez F.

BMC Biotechnol. 2009 Feb 26;9:14. doi: 10.1186/1472-6750-9-14.

17.

A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220.

Yang T, Yang L, Chai W, Li R, Xie J, Niu B.

Protein Expr Purif. 2011 Mar;76(1):109-14. doi: 10.1016/j.pep.2010.10.006. Epub 2010 Oct 14.

PMID:
20951213
18.

Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.

Yuasa N, Zhang W, Goto T, Sakaue H, Matsumoto-Takasaki A, Kimura M, Ohshima H, Tsuchida Y, Koizumi T, Sakai K, Kojima T, Yamamoto K, Nakata M, Fujita-Yamaguchi Y.

J Biol Chem. 2010 Oct 1;285(40):30587-97. doi: 10.1074/jbc.M110.107284. Epub 2010 Jul 28.

19.

An efficient strategy for cell-based antibody library selection using an integrated vector system.

Yoon H, Song JM, Ryu CJ, Kim YG, Lee EK, Kang S, Kim SJ.

BMC Biotechnol. 2012 Sep 18;12:62. doi: 10.1186/1472-6750-12-62.

20.

Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.

Kubota T, Matsushita T, Niwa R, Kumagai I, Nakamura K.

Anticancer Res. 2010 Sep;30(9):3397-405.

PMID:
20944114
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