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Items: 1 to 20 of 116

1.

AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications.

Huang X, Hartley AV, Yin Y, Herskowitz JH, Lah JJ, Ressler KJ.

J Virol Methods. 2013 Nov;193(2):270-7. doi: 10.1016/j.jviromet.2013.06.008. Epub 2013 Jun 19.

2.

Rapid, simple, and versatile manufacturing of recombinant adeno-associated viral vectors at scale.

Lock M, Alvira M, Vandenberghe LH, Samanta A, Toelen J, Debyser Z, Wilson JM.

Hum Gene Ther. 2010 Oct;21(10):1259-71. doi: 10.1089/hum.2010.055.

3.

Scalable serum-free production of recombinant adeno-associated virus type 2 by transfection of 293 suspension cells.

Durocher Y, Pham PL, St-Laurent G, Jacob D, Cass B, Chahal P, Lau CJ, Nalbantoglu J, Kamen A.

J Virol Methods. 2007 Sep;144(1-2):32-40. Epub 2007 Apr 30.

PMID:
17467815
4.

Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery.

Chahal PS, Schulze E, Tran R, Montes J, Kamen AA.

J Virol Methods. 2014 Feb;196:163-73. doi: 10.1016/j.jviromet.2013.10.038. Epub 2013 Nov 13.

PMID:
24239634
5.

Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors.

Reed SE, Staley EM, Mayginnes JP, Pintel DJ, Tullis GE.

J Virol Methods. 2006 Dec;138(1-2):85-98. Epub 2006 Sep 6.

PMID:
16950522
6.

High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap.

Conway JE, Rhys CM, Zolotukhin I, Zolotukhin S, Muzyczka N, Hayward GS, Byrne BJ.

Gene Ther. 1999 Jun;6(6):986-93.

7.

Optimization of 25 kDa linear polyethylenimine for efficient gene delivery.

Huh SH, Do HJ, Lim HY, Kim DK, Choi SJ, Song H, Kim NH, Park JK, Chang WK, Chung HM, Kim JH.

Biologicals. 2007 Jun;35(3):165-71. Epub 2006 Nov 1.

PMID:
17084092
8.
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10.

Scalable production of adeno-associated virus type 2 vectors via suspension transfection.

Park JY, Lim BP, Lee K, Kim YG, Jo EC.

Biotechnol Bioeng. 2006 Jun 20;94(3):416-30.

PMID:
16622883
11.

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells.

Hildinger M, Baldi L, Stettler M, Wurm FM.

Biotechnol Lett. 2007 Nov;29(11):1713-21. Epub 2007 Jul 17.

PMID:
17636388
12.

Factors influencing recombinant adeno-associated virus production.

Salvetti A, Orève S, Chadeuf G, Favre D, Cherel Y, Champion-Arnaud P, David-Ameline J, Moullier P.

Hum Gene Ther. 1998 Mar 20;9(5):695-706.

PMID:
9551617
13.

AAV2/8 vectors purified from culture medium with a simple and rapid protocol transduce murine liver, muscle, and retina efficiently.

Doria M, Ferrara A, Auricchio A.

Hum Gene Ther Methods. 2013 Dec;24(6):392-8. doi: 10.1089/hgtb.2013.155. Epub 2013 Oct 11.

14.
15.

A novel polyethyleneimine-coated adeno-associated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: preparation and in vitro analysis.

Shao W, Paul A, Abbasi S, Chahal PS, Mena JA, Montes J, Kamen A, Prakash S.

Int J Nanomedicine. 2012;7:1575-86. doi: 10.2147/IJN.S26891. Epub 2012 Mar 23.

16.

Lipoic acid modified low molecular weight polyethylenimine mediates nontoxic and highly potent in vitro gene transfection.

Zheng M, Zhong Y, Meng F, Peng R, Zhong Z.

Mol Pharm. 2011 Dec 5;8(6):2434-43. doi: 10.1021/mp2003797. Epub 2011 Sep 28.

PMID:
21923163
17.

Non-viral gene transfection in vitro using endosomal pH-sensitive reversibly hydrophobilized polyethylenimine.

Liu Z, Zheng M, Meng F, Zhong Z.

Biomaterials. 2011 Dec;32(34):9109-19. doi: 10.1016/j.biomaterials.2011.08.017. Epub 2011 Sep 3.

PMID:
21890198
18.

Optimization of recombinant adeno-associated virus production using an herpes simplex virus amplicon system.

Feudner E, de Alwis M, Thrasher AJ, Ali RR, Fauser S.

J Virol Methods. 2001 Aug;96(2):97-105.

PMID:
11445141
19.

Production and discovery of novel recombinant adeno-associated viral vectors.

Mueller C, Ratner D, Zhong L, Esteves-Sena M, Gao G.

Curr Protoc Microbiol. 2012 Aug;Chapter 14:Unit14D.1. doi: 10.1002/9780471729259.mc14d01s26.

20.

An efficient and scalable process for helper-dependent adenoviral vector production using polyethylenimine-adenofection.

Dormond E, Meneses-Acosta A, Jacob D, Durocher Y, Gilbert R, Perrier M, Kamen A.

Biotechnol Bioeng. 2009 Feb 15;102(3):800-10. doi: 10.1002/bit.22113.

PMID:
18821637
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