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Items: 1 to 20 of 106

1.

Expression analysis of rare cellular subsets: direct RT-PCR on limited cell numbers obtained by FACS or soft agar assays.

Ho V, Yeo SY, Kunasegaran K, De Silva D, Tarulli GA, Voorhoeve PM, Pietersen AM.

Biotechniques. 2013 Apr;54(4):208-12. doi: 10.2144/000114019.

2.

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.

Bengtsson M, Hemberg M, Rorsman P, Ståhlberg A.

BMC Mol Biol. 2008 Jul 17;9:63. doi: 10.1186/1471-2199-9-63.

3.

Quantitative single cell gene expression profiling in the avian embryo.

Morrison JA, Box AC, McKinney MC, McLennan R, Kulesa PM.

Dev Dyn. 2015 Jun;244(6):774-84. doi: 10.1002/dvdy.24274.

PMID:
25809747
4.

Quantification of chemotherapeutic target gene mRNA expression in human breast cancer biopsies: comparison of real-time reverse transcription-PCR vs. relative quantification reverse transcription-PCR utilizing DNA sequencer analysis of PCR products.

Juhasz A, Frankel P, Cheng C, Rivera H, Vishwanath R, Chiu A, Margolin K, Yen Y, Newman EM, Synold T, Wilczynski S, Lenz HJ, Gandara D, Albain KS, Longmate J, Doroshow JH.

J Clin Lab Anal. 2003;17(5):184-94.

PMID:
12938148
6.

[Use of the real-time RT-PCR method for investigation of small stable RNA expression level in human epidermoid carcinoma cells A431].

Nikitina TV, Nazarova NIu, Tishchenko LI, Tuohimaa P, Sedova VM.

Tsitologiia. 2003;45(4):392-402. Russian.

PMID:
14520871
7.

Regulator of G-protein signalling 2 mRNA is differentially expressed in mammary epithelial subpopulations and over-expressed in the majority of breast cancers.

Smalley MJ, Iravani M, Leao M, Grigoriadis A, Kendrick H, Dexter T, Fenwick K, Regan JL, Britt K, McDonald S, Lord CJ, Mackay A, Ashworth A.

Breast Cancer Res. 2007;9(6):R85.

8.

Transcriptome analysis of single cells.

Morris J, Singh JM, Eberwine JH.

J Vis Exp. 2011 Apr 25;(50). pii: 2634. doi: 10.3791/2634. Erratum in: J Vis Exp. 2011;57. doi: 10.3791/4082.

9.

Quantitative analysis of specific mRNA species in minute cell samples by RT-PCR and flow cytometry.

Stemme V, Rymo L, Risberg B, Stemme S.

J Immunol Methods. 2001 Mar 1;249(1-2):223-33.

PMID:
11226479
10.

Single-cell gene expression profiling using reverse transcription quantitative real-time PCR.

Ståhlberg A, Bengtsson M.

Methods. 2010 Apr;50(4):282-8. doi: 10.1016/j.ymeth.2010.01.002. Epub 2010 Jan 11. Review.

PMID:
20064613
11.

Differential expression of WISP-1 and WISP-2 genes in normal and transformed human breast cell lines.

Saxena N, Banerjee S, Sengupta K, Zoubine MN, Banerjee SK.

Mol Cell Biochem. 2001 Dec;228(1-2):99-104.

PMID:
11855747
12.

Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos.

Hartshorn C, Eckert JJ, Hartung O, Wangh LJ.

BMC Biotechnol. 2007 Dec 7;7:87.

13.

CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.

Boeuf P, Vigan-Womas I, Jublot D, Loizon S, Barale JC, Akanmori BD, Mercereau-Puijalon O, Behr C.

BMC Immunol. 2005 Mar 4;6:5.

14.

Cryopreserved human B cells as an alternative source for single cell mRNA analysis.

Hansen A, Reiter K, Dörner T, Pruss A.

Cell Tissue Bank. 2005;6(4):299-308.

PMID:
16308769
15.

Overexpression and activation of the alpha9-nicotinic receptor during tumorigenesis in human breast epithelial cells.

Lee CH, Huang CS, Chen CS, Tu SH, Wang YJ, Chang YJ, Tam KW, Wei PL, Cheng TC, Chu JS, Chen LC, Wu CH, Ho YS.

J Natl Cancer Inst. 2010 Sep 8;102(17):1322-35. doi: 10.1093/jnci/djq300. Epub 2010 Aug 23.

16.

Profiling of gene expression in individual hematopoietic cells by global mRNA amplification and slot blot analysis.

Theilgaard-Mönch K, Cowland J, Borregaard N.

J Immunol Methods. 2001 Jun 1;252(1-2):175-89.

PMID:
11334978
17.

Real-time PCR analysis for blood cell lineage specific markers.

Edvardsson L, Olofsson T.

Methods Mol Biol. 2009;496:313-22. doi: 10.1007/978-1-59745-553-4_21. Review.

PMID:
18839120
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20.

Expression profiling of glycosyltransferases using RT-PCR.

Kobayashi M.

Methods Enzymol. 2006;416:129-40.

PMID:
17113864
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