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Results: 1 to 20 of 104

1.

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.

Lin-Cereghino GP, Stark CM, Kim D, Chang J, Shaheen N, Poerwanto H, Agari K, Moua P, Low LK, Tran N, Huang AD, Nattestad M, Oshiro KT, Chang JW, Chavan A, Tsai JW, Lin-Cereghino J.

Gene. 2013 May 1;519(2):311-7. doi: 10.1016/j.gene.2013.01.062. Epub 2013 Feb 21.

PMID:
23454485
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

[Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris].

Xiong AS, Peng RH, Li X, Fan HQ, Yao QH, Guo MJ, Zhang SL.

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Feb;35(2):154-60. Chinese.

PMID:
12545223
[PubMed - indexed for MEDLINE]
3.

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris.

Liang S, Li C, Ye Y, Lin Y.

Biotechnol Lett. 2013 Jan;35(1):97-105. doi: 10.1007/s10529-012-1055-8. Epub 2012 Sep 25.

PMID:
23160737
[PubMed - indexed for MEDLINE]
4.

High-yield secretion of recombinant gelatins by Pichia pastoris.

Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, de Wolf FA.

Yeast. 1999 Aug;15(11):1087-96.

PMID:
10455232
[PubMed - indexed for MEDLINE]
5.

Secretory expression and characterization of insulin in Pichia pastoris.

Kjeldsen T, Pettersson AF, Hach M.

Biotechnol Appl Biochem. 1999 Feb;29 ( Pt 1):79-86.

PMID:
9889087
[PubMed - indexed for MEDLINE]
6.

Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris.

Vadhana AK, Samuel P, Berin RM, Krishna J, Kamatchi K, Meenakshisundaram S.

Enzyme Microb Technol. 2013 Mar 5;52(3):177-83. doi: 10.1016/j.enzmictec.2013.01.001. Epub 2013 Jan 7.

PMID:
23410929
[PubMed - indexed for MEDLINE]
7.

Efficient expression, purification and characterization of mouse salivary alpha-amylase secreted from methylotrophic yeast, Pichia pastoris.

Kato S, Ishibashi M, Tatsuda D, Tokunaga H, Tokunaga M.

Yeast. 2001 May;18(7):643-55.

PMID:
11329174
[PubMed - indexed for MEDLINE]
8.

Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris.

Staley CA, Huang A, Nattestad M, Oshiro KT, Ray LE, Mulye T, Li ZH, Le T, Stephens JJ, Gomez SR, Moy AD, Nguyen JC, Franz AH, Lin-Cereghino J, Lin-Cereghino GP.

Gene. 2012 Apr 1;496(2):118-27. doi: 10.1016/j.gene.2012.01.008. Epub 2012 Jan 25.

PMID:
22285974
[PubMed - indexed for MEDLINE]
Free PMC Article
9.

Human lysozyme secretion increased by alpha-factor pro-sequence in Pichia pastoris.

Oka C, Tanaka M, Muraki M, Harata K, Suzuki K, Jigami Y.

Biosci Biotechnol Biochem. 1999 Nov;63(11):1977-83.

PMID:
10635562
[PubMed - indexed for MEDLINE]
Free Article
10.

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

Martínez-Ruiz A, Martínez del Pozo A, Lacadena J, Mancheño JM, Oñaderra M, López-otín C, Gavilanes JG.

Protein Expr Purif. 1998 Apr;12(3):315-22.

PMID:
9535698
[PubMed - indexed for MEDLINE]
11.

Mutagenesis of key amino acids alters activity of a Saccharomyces cerevisiae endo-polygalacturonase expressed in Pichia pastoris.

Blanco P, Thow G, Simpson CG, Villa TG, Williamson B.

FEMS Microbiol Lett. 2002 May 7;210(2):187-91.

PMID:
12044673
[PubMed - indexed for MEDLINE]
12.

Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence.

Eilert E, Rolf T, Heumaier A, Hollenberg CP, Piontek M, Suckow M.

J Biotechnol. 2013 Aug 20;167(2):94-100. doi: 10.1016/j.jbiotec.2012.08.024. Epub 2012 Sep 7.

PMID:
22982399
[PubMed - indexed for MEDLINE]
13.

High-level expression of Candida parapsilosis lipase/acyltransferase in Pichia pastoris.

Brunel L, Neugnot V, Landucci L, Boze H, Moulin G, Bigey F, Dubreucq E.

J Biotechnol. 2004 Jul 1;111(1):41-50.

PMID:
15196768
[PubMed - indexed for MEDLINE]
14.

Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris.

Gu L, Lajoie C, Kelly C.

Biotechnol Prog. 2003 Sep-Oct;19(5):1403-9.

PMID:
14524699
[PubMed - indexed for MEDLINE]
15.

Comparison of two expression platforms in respect to protein yield and quality: Pichia pastoris versus Pichia angusta.

Mack M, Wannemacher M, Hobl B, Pietschmann P, Hock B.

Protein Expr Purif. 2009 Aug;66(2):165-71. doi: 10.1016/j.pep.2009.03.010. Epub 2009 Apr 7.

PMID:
19358890
[PubMed - indexed for MEDLINE]
16.

High-level secretion of biologically active aprotinin from the yeast Pichia pastoris.

Vedvick T, Buckholz RG, Engel M, Urcan M, Kinney J, Provow S, Siegel RS, Thill GP.

J Ind Microbiol. 1991 Apr;7(3):197-201.

PMID:
1370032
[PubMed - indexed for MEDLINE]
17.

Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe.

Eiden-Plach A, Zagorc T, Heintel T, Carius Y, Breinig F, Schmitt MJ.

Appl Environ Microbiol. 2004 Feb;70(2):961-6.

PMID:
14766577
[PubMed - indexed for MEDLINE]
Free PMC Article
18.

Expression and characterization of human interferon-beta1 in the methylotrophic yeast Pichia pastoris.

Skoko N, Argamante B, Grujicić NK, Tisminetzky SG, Glisin V, Ljubijankić G.

Biotechnol Appl Biochem. 2003 Dec;38(Pt 3):257-65.

PMID:
12911337
[PubMed - indexed for MEDLINE]
19.

Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies.

Clare JJ, Romanos MA, Rayment FB, Rowedder JE, Smith MA, Payne MM, Sreekrishna K, Henwood CA.

Gene. 1991 Sep 15;105(2):205-12.

PMID:
1937016
[PubMed - indexed for MEDLINE]
20.

Expression, characterization, and purification of recombinant porcine lactoferrin in Pichia pastoris.

Wang SH, Yang TS, Lin SM, Tsai MS, Wu SC, Mao SJ.

Protein Expr Purif. 2002 Jun;25(1):41-9.

PMID:
12071697
[PubMed - indexed for MEDLINE]

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