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Items: 1 to 20 of 98


A do-it-yourself protocol for simple transcription activator-like effector assembly.

Uhde-Stone C, Gor N, Chin T, Huang J, Lu B.

Biol Proced Online. 2013 Jan 14;15(1):3. doi: 10.1186/1480-9222-15-3.


A transcription activator-like effector toolbox for genome engineering.

Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F.

Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.


FusX: A Rapid One-Step Transcription Activator-Like Effector Assembly System for Genome Science.

Ma AC, McNulty MS, Poshusta TL, Campbell JM, Martínez-Gálvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC.

Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172. Epub 2016 Mar 15.


Engineering customized TALE nucleases (TALENs) and TALE transcription factors by fast ligation-based automatable solid-phase high-throughput (FLASH) assembly.

Reyon D, Maeder ML, Khayter C, Tsai SQ, Foley JE, Sander JD, Joung JK.

Curr Protoc Mol Biol. 2013 Jul;Chapter 12:Unit 12.16. doi: 10.1002/0471142727.mb1216s103.


A simple and efficient method for assembling TALE protein based on plasmid library.

Zhang Z, Li D, Xu H, Xin Y, Zhang T, Ma L, Wang X, Chen Z, Zhang Z.

PLoS One. 2013 Jun 20;8(6):e66459. doi: 10.1371/journal.pone.0066459. Print 2013.


Efficient design and assembly of custom TALENs using the Golden Gate platform.

Cermak T, Starker CG, Voytas DF.

Methods Mol Biol. 2015;1239:133-59. doi: 10.1007/978-1-4939-1862-1_7.


TALE: a tale of genome editing.

Zhang M, Wang F, Li S, Wang Y, Bai Y, Xu X.

Prog Biophys Mol Biol. 2014 Jan;114(1):25-32. doi: 10.1016/j.pbiomolbio.2013.11.006. Epub 2013 Nov 27. Review.


Rapid and efficient assembly of transcription activator-like effector genes by USER cloning.

Wang S, Li W, Wang S, Hu B.

J Genet Genomics. 2014 Jun 20;41(6):339-47. doi: 10.1016/j.jgg.2014.05.002. Epub 2014 May 17.


The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified.

Zheng CK, Wang CL, Zhang XP, Wang FJ, Qin TF, Zhao KJ.

Mol Plant Pathol. 2014 Sep;15(7):690-7. doi: 10.1111/mpp.12125. Epub 2014 Apr 10.


Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.

Thakore PI, Gersbach CA.

Methods Mol Biol. 2016;1338:71-88. doi: 10.1007/978-1-4939-2932-0_7.


Rapid and highly efficient construction of TALE-based transcriptional regulators and nucleases for genome modification.

Li L, Piatek MJ, Atef A, Piatek A, Wibowo A, Fang X, Sabir JS, Zhu JK, Mahfouz MM.

Plant Mol Biol. 2012 Mar;78(4-5):407-16. doi: 10.1007/s11103-012-9875-4. Epub 2012 Jan 22.


Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos.

Lei Y, Guo X, Deng Y, Chen Y, Zhao H.

Cell Biosci. 2013 May 10;3(1):21. doi: 10.1186/2045-3701-3-21.


Rapid assembly of customized TALENs into multiple delivery systems.

Zhang Z, Zhang S, Huang X, Orwig KE, Sheng Y.

PLoS One. 2013 Nov 7;8(11):e80281. doi: 10.1371/journal.pone.0080281. eCollection 2013.


A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity.

Mussolino C, Morbitzer R, Lütge F, Dannemann N, Lahaye T, Cathomen T.

Nucleic Acids Res. 2011 Nov;39(21):9283-93. doi: 10.1093/nar/gkr597. Epub 2011 Aug 3.


Assembly of custom TALE-type DNA binding domains by modular cloning.

Morbitzer R, Elsaesser J, Hausner J, Lahaye T.

Nucleic Acids Res. 2011 Jul;39(13):5790-9. doi: 10.1093/nar/gkr151. Epub 2011 Mar 18.


TAL effector nuclease (TALEN) engineering.

Li T, Yang B.

Methods Mol Biol. 2013;978:63-72. doi: 10.1007/978-1-62703-293-3_5.


A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors.

Uhde-Stone C, Huang J, Lu B.

Biol Proced Online. 2012 Aug 8;14(1):8. doi: 10.1186/1480-9222-14-8.


ULtiMATE system for rapid assembly of customized TAL effectors.

Yang J, Yuan P, Wen D, Sheng Y, Zhu S, Yu Y, Gao X, Wei W.

PLoS One. 2013 Sep 27;8(9):e75649. doi: 10.1371/journal.pone.0075649. eCollection 2013.


Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing.

Sun N, Zhao H.

Biotechnol Bioeng. 2013 Jul;110(7):1811-21. doi: 10.1002/bit.24890. Epub 2013 Apr 7. Review.


Efficient TALEN construction and evaluation methods for human cell and animal applications.

Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T.

Genes Cells. 2013 Apr;18(4):315-26. doi: 10.1111/gtc.12037. Epub 2013 Feb 6.

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