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Results: 1 to 20 of 103

1.

Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.

Leibly DJ, Nguyen TN, Kao LT, Hewitt SN, Barrett LK, Van Voorhis WC.

PLoS One. 2012;7(12):e52482. doi: 10.1371/journal.pone.0052482. Epub 2012 Dec 20.

PMID:
23285060
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli.

Patra AK, Mukhopadhyay R, Mukhija R, Krishnan A, Garg LC, Panda AK.

Protein Expr Purif. 2000 Mar;18(2):182-92.

PMID:
10686149
[PubMed - indexed for MEDLINE]
3.

Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol.

Kumar A, Tiwari S, Thavaselvam D, Sathyaseelan K, Prakash A, Barua A, Arora S, Kameswara Rao M.

Protein Expr Purif. 2012 Jun;83(2):226-32. doi: 10.1016/j.pep.2012.04.002. Epub 2012 Apr 21.

PMID:
22542588
[PubMed - indexed for MEDLINE]
4.

Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.

Tsumoto K, Umetsu M, Kumagai I, Ejima D, Arakawa T.

Biochem Biophys Res Commun. 2003 Dec 26;312(4):1383-6.

PMID:
14652027
[PubMed - indexed for MEDLINE]
5.

The transcriptional response of Escherichia coli to recombinant protein insolubility.

Smith HE.

J Struct Funct Genomics. 2007 Mar;8(1):27-35. Epub 2007 Nov 9.

PMID:
17992580
[PubMed - indexed for MEDLINE]
6.
7.

Renaturation, purification and characterization of streptokinase expressed as inclusion body in recombinant E. coli.

Cherish Babu PV, Srinivas VK, Krishna Mohan V, Krishna E.

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 15;861(2):218-26. Epub 2007 Oct 13.

PMID:
17981102
[PubMed - indexed for MEDLINE]
8.

Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.

Quartley E, Alexandrov A, Mikucki M, Buckner FS, Hol WG, DeTitta GT, Phizicky EM, Grayhack EJ.

J Struct Funct Genomics. 2009 Sep;10(3):233-47. doi: 10.1007/s10969-009-9068-9. Epub 2009 Aug 22.

PMID:
19701618
[PubMed - indexed for MEDLINE]
9.

Thermofluor-based high-throughput stability optimization of proteins for structural studies.

Ericsson UB, Hallberg BM, Detitta GT, Dekker N, Nordlund P.

Anal Biochem. 2006 Oct 15;357(2):289-98. Epub 2006 Aug 10.

PMID:
16962548
[PubMed - indexed for MEDLINE]
10.

[Influence of the reductase deficient Escherichia coli on the solubility of recombinant proteins produced in it].

Xiong S, Zhang MY, Qian CW, Ran YC, Wang YF, Ren XR, Su KY, Yu ZY.

Sheng Wu Gong Cheng Xue Bao. 2003 Nov;19(6):686-91. Chinese.

PMID:
15971580
[PubMed - indexed for MEDLINE]
11.

Solubilization and refolding with simultaneous purification of recombinant human stem cell factor.

Wang C, Liu J, Wang L, Geng X.

Appl Biochem Biotechnol. 2008 Feb;144(2):181-9.

PMID:
18456949
[PubMed - indexed for MEDLINE]
12.

Role of arginine in protein refolding, solubilization, and purification.

Tsumoto K, Umetsu M, Kumagai I, Ejima D, Philo JS, Arakawa T.

Biotechnol Prog. 2004 Sep-Oct;20(5):1301-8. Review.

PMID:
15458311
[PubMed - indexed for MEDLINE]
13.

A sparse matrix approach to the solubilization of overexpressed proteins.

Lindwall G, Chau M, Gardner SR, Kohlstaedt LA.

Protein Eng. 2000 Jan;13(1):67-71.

PMID:
10679532
[PubMed - indexed for MEDLINE]
Free Article
14.

Effect of chemical chaperones in improving the solubility of recombinant proteins in Escherichia coli.

Prasad S, Khadatare PB, Roy I.

Appl Environ Microbiol. 2011 Jul;77(13):4603-9. doi: 10.1128/AEM.05259-11. Epub 2011 May 6.

PMID:
21551288
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

Identifying solubility-promoting buffers for intrinsically disordered proteins prior to purification.

Churion KA, Bondos SE.

Methods Mol Biol. 2012;896:415-27. doi: 10.1007/978-1-4614-3704-8_28.

PMID:
22821541
[PubMed - indexed for MEDLINE]
16.

Refolding solubilized inclusion body proteins.

Burgess RR.

Methods Enzymol. 2009;463:259-82. doi: 10.1016/S0076-6879(09)63017-2. Review.

PMID:
19892177
[PubMed - indexed for MEDLINE]
17.

Estimating the potential refolding yield of recombinant proteins expressed as inclusion bodies.

Ho JG, Middelberg AP.

Biotechnol Bioeng. 2004 Sep 5;87(5):584-92.

PMID:
15352056
[PubMed - indexed for MEDLINE]
18.

Purification and characterization of human and mouse recombinant alpha-fetoproteins expressed in Escherichia coli.

Boismenu R, Semeniuk D, Murgita RA.

Protein Expr Purif. 1997 Jun;10(1):10-26.

PMID:
9179285
[PubMed - indexed for MEDLINE]
19.

Efficient solubilization of inclusion bodies.

Freydell EJ, Ottens M, Eppink M, van Dedem G, van der Wielen L.

Biotechnol J. 2007 Jun;2(6):678-84.

PMID:
17492713
[PubMed - indexed for MEDLINE]
20.

Solubilization and refolding of bacterial inclusion body proteins.

Singh SM, Panda AK.

J Biosci Bioeng. 2005 Apr;99(4):303-10. Review.

PMID:
16233795
[PubMed - indexed for MEDLINE]
Free Article

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