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Results: 1 to 20 of 97

1.

Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.

Encell LP, Friedman Ohana R, Zimmerman K, Otto P, Vidugiris G, Wood MG, Los GV, McDougall MG, Zimprich C, Karassina N, Learish RD, Hurst R, Hartnett J, Wheeler S, Stecha P, English J, Zhao K, Mendez J, Benink HA, Murphy N, Daniels DL, Slater MR, Urh M, Darzins A, Klaubert DH, Bulleit RF, Wood KV.

Curr Chem Genomics. 2012;6:55-71. doi: 10.2174/1875397301206010055. Epub 2012 Oct 5.

PMID:
23248739
[PubMed]
Free PMC Article
2.

The HaloTag: Improving Soluble Expression and Applications in Protein Functional Analysis.

N Peterson S, Kwon K.

Curr Chem Genomics. 2012;6:8-17. doi: 10.2174/1875397301206010008. Epub 2012 Sep 20.

PMID:
23115610
[PubMed]
Free PMC Article
3.

HaloTag-based purification of functional human kinases from mammalian cells.

Ohana RF, Hurst R, Vidugiriene J, Slater MR, Wood KV, Urh M.

Protein Expr Purif. 2011 Apr;76(2):154-64. doi: 10.1016/j.pep.2010.11.014. Epub 2010 Dec 1.

PMID:
21129486
[PubMed - indexed for MEDLINE]
4.

HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, Wood MG, Learish R, Ohana RF, Urh M, Simpson D, Mendez J, Zimmerman K, Otto P, Vidugiris G, Zhu J, Darzins A, Klaubert DH, Bulleit RF, Wood KV.

ACS Chem Biol. 2008 Jun 20;3(6):373-82. doi: 10.1021/cb800025k.

PMID:
18533659
[PubMed - indexed for MEDLINE]
5.

Orthogonal protein purification facilitated by a small bispecific affinity tag.

Nilvebrant J, Alm T, Hober S.

J Vis Exp. 2012 Jan 16;(59). pii: 3370. doi: 10.3791/3370.

PMID:
22297419
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins.

Gaj T, Meyer SC, Ghosh I.

Protein Expr Purif. 2007 Nov;56(1):54-61. Epub 2007 Jul 30.

PMID:
17697784
[PubMed - indexed for MEDLINE]
7.

Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins.

Tirat A, Freuler F, Stettler T, Mayr LM, Leder L.

Int J Biol Macromol. 2006 Aug 15;39(1-3):66-76. Epub 2006 Feb 28.

PMID:
16503347
[PubMed - indexed for MEDLINE]
8.

Methods for detection of protein-protein and protein-DNA interactions using HaloTag.

Urh M, Hartzell D, Mendez J, Klaubert DH, Wood K.

Methods Mol Biol. 2008;421:191-209.

PMID:
18826056
[PubMed - indexed for MEDLINE]
9.
10.

HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.

Ohana RF, Encell LP, Zhao K, Simpson D, Slater MR, Urh M, Wood KV.

Protein Expr Purif. 2009 Nov;68(1):110-20. doi: 10.1016/j.pep.2009.05.010. Epub 2009 May 21.

PMID:
19464373
[PubMed - indexed for MEDLINE]
11.

Commonly used tag combinations for tandem affinity purification.

Li Y.

Biotechnol Appl Biochem. 2010 Feb 15;55(2):73-83. doi: 10.1042/BA20090273. Review.

PMID:
20156193
[PubMed - indexed for MEDLINE]
12.

Site-specific, covalent labeling of recombinant antibody fragments via fusion to an engineered version of 6-O-alkylguanine DNA alkyltransferase.

Kampmeier F, Ribbert M, Nachreiner T, Dembski S, Beaufils F, Brecht A, Barth S.

Bioconjug Chem. 2009 May 20;20(5):1010-5. doi: 10.1021/bc9000257.

PMID:
19388673
[PubMed - indexed for MEDLINE]
13.

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.

Arnau J, Lauritzen C, Petersen GE, Pedersen J.

Protein Expr Purif. 2006 Jul;48(1):1-13. Epub 2005 Dec 28. Review.

PMID:
16427311
[PubMed - indexed for MEDLINE]
14.

Affinity tag for protein purification and detection based on the disulfide-linked complex of InaD and NorpA.

Kimple ME, Sondek J.

Biotechniques. 2002 Sep;33(3):578, 580, 584-8 passim.

PMID:
12238768
[PubMed - indexed for MEDLINE]
15.

A "tag-and-modify" approach to site-selective protein modification.

Chalker JM, Bernardes GJ, Davis BG.

Acc Chem Res. 2011 Sep 20;44(9):730-41. doi: 10.1021/ar200056q. Epub 2011 May 12.

PMID:
21563755
[PubMed - indexed for MEDLINE]
16.

Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins.

Routzahn KM, Waugh DS.

J Struct Funct Genomics. 2002;2(2):83-92.

PMID:
12836665
[PubMed - indexed for MEDLINE]
17.

A tailor-made "tag-receptor" affinity pair for the purification of fusion proteins.

Pina AS, Guilherme M, Pereira AS, Fernandes CS, Branco RJ, El Khoury G, Lowe CR, Roque AC.

Chembiochem. 2014 Jul 7;15(10):1423-35. doi: 10.1002/cbic.201400018. Epub 2014 Jun 5.

PMID:
24903894
[PubMed - in process]
18.

A PCR-directed cell-free approach to optimize protein expression using diverse fusion tags.

Kralicek AV, Radjainia M, Mohamad Ali NA, Carraher C, Newcomb RD, Mitra AK.

Protein Expr Purif. 2011 Nov;80(1):117-24. doi: 10.1016/j.pep.2011.06.006. Epub 2011 Jun 22.

PMID:
21722735
[PubMed - indexed for MEDLINE]
19.

New chemistry for the study of multiprotein complexes: the six-histidine tag as a receptor for a protein crosslinking reagent.

Fancy DA, Melcher K, Johnston SA, Kodadek T.

Chem Biol. 1996 Jul;3(7):551-9.

PMID:
8807887
[PubMed - indexed for MEDLINE]
20.

Reprint of: Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.

Arnau J, Lauritzen C, Petersen GE, Pedersen J.

Protein Expr Purif. 2011 Sep 2. [Epub ahead of print]

PMID:
21889989
[PubMed - as supplied by publisher]

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