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Results: 1 to 20 of 103

1.

Quantitative real-time PCR as a sensitive protein-protein interaction quantification method and a partial solution for non-accessible autoactivator and false-negative molecule analysis in the yeast two-hybrid system.

Maier RH, Maier CJ, Hintner H, Bauer JW, Onder K.

Methods. 2012 Dec;58(4):376-84. doi: 10.1016/j.ymeth.2012.09.001. Epub 2012 Sep 13.

PMID:
22982175
[PubMed - indexed for MEDLINE]
2.

Relative quantitation of protein-protein interaction strength within the yeast two-hybrid system via fluorescence beta-galactosidase activity detection in a high-throughput and low-cost manner.

Oender K, Niedermayr P, Hintner H, Richter K, Koller L, Trost A, Bauer JW, Hundsberger H.

Assay Drug Dev Technol. 2006 Dec;4(6):709-19.

PMID:
17199509
[PubMed - indexed for MEDLINE]
3.

Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent and site-specific recombinational cloning vector system.

Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K.

Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 10.1089/adt.2009.0266. Epub 2010 Jun 1.

PMID:
20515414
[PubMed - indexed for MEDLINE]
4.

A comparison and optimization of yeast two-hybrid systems.

Caufield JH, Sakhawalkar N, Uetz P.

Methods. 2012 Dec;58(4):317-24. doi: 10.1016/j.ymeth.2012.12.001. Epub 2012 Dec 8.

PMID:
23231818
[PubMed - indexed for MEDLINE]
5.

A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping.

Chen J, Zhou J, Sanders CK, Nolan JP, Cai H.

Anal Biochem. 2009 Jul 1;390(1):29-37. doi: 10.1016/j.ab.2009.03.013. Epub 2009 Mar 17.

PMID:
19298787
[PubMed - indexed for MEDLINE]
6.

Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter.

Kamiya T, Ojima T, Sugimoto K, Nakano H, Kawarasaki Y.

J Biotechnol. 2010 Apr 15;146(4):151-9. doi: 10.1016/j.jbiotec.2010.02.007. Epub 2010 Feb 18.

PMID:
20171251
[PubMed - indexed for MEDLINE]
7.

Construction of a reading frame-independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening.

Maier R, Brandner C, Hintner H, Bauer J, Onder K.

Biotechniques. 2008 Sep;45(3):235-44. doi: 10.2144/000112897.

PMID:
18778248
[PubMed - indexed for MEDLINE]
Free Article
8.

A yEGFP-based reporter system for high-throughput yeast two-hybrid assay by flow cytometry.

Chen J, Zhou J, Bae W, Sanders CK, Nolan JP, Cai H.

Cytometry A. 2008 Apr;73(4):312-20. doi: 10.1002/cyto.a.20525.

PMID:
18307271
[PubMed - indexed for MEDLINE]
Free Article
9.

Increasing specificity in high-throughput yeast two-hybrid experiments.

Vidalain PO, Boxem M, Ge H, Li S, Vidal M.

Methods. 2004 Apr;32(4):363-70.

PMID:
15003598
[PubMed - indexed for MEDLINE]
10.

Yeast two-hybrid screening of cyclic peptide libraries using a combination of random and PI-deconvolution pooling strategies.

Barreto K, Aparicio A, Bharathikumar VM, DeCoteau JF, Geyer CR.

Protein Eng Des Sel. 2012 Sep;25(9):453-64. doi: 10.1093/protein/gzs029. Epub 2012 Jul 4.

PMID:
22763264
[PubMed - indexed for MEDLINE]
Free Article
11.

Analysis of protein-protein interactions using high-throughput yeast two-hybrid screens.

Rajagopala SV, Uetz P.

Methods Mol Biol. 2011;781:1-29. doi: 10.1007/978-1-61779-276-2_1.

PMID:
21877274
[PubMed - indexed for MEDLINE]
12.

Fifteen years of the yeast three-hybrid system: RNA-protein interactions under investigation.

Martin F.

Methods. 2012 Dec;58(4):367-75. doi: 10.1016/j.ymeth.2012.07.016. Epub 2012 Jul 24. Review.

PMID:
22841566
[PubMed - indexed for MEDLINE]
13.

Yeast protein-protein interaction assays and screens.

de Folter S, Immink RG.

Methods Mol Biol. 2011;754:145-65. doi: 10.1007/978-1-61779-154-3_8.

PMID:
21720951
[PubMed - indexed for MEDLINE]
14.

An integrated approach to elucidate the intra-viral and viral-cellular protein interaction networks of a gamma-herpesvirus.

Lee S, Salwinski L, Zhang C, Chu D, Sampankanpanich C, Reyes NA, Vangeloff A, Xing F, Li X, Wu TT, Sahasrabudhe S, Deng H, Lacount DJ, Sun R.

PLoS Pathog. 2011 Oct;7(10):e1002297. doi: 10.1371/journal.ppat.1002297. Epub 2011 Oct 20.

PMID:
22028648
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

Construction of improved Yeast Two-Hybrid libraries.

Maier RH, Maier CJ, Onder K.

Methods Mol Biol. 2011;729:71-84. doi: 10.1007/978-1-61779-065-2_5.

PMID:
21365484
[PubMed - indexed for MEDLINE]
16.

A novel scoring approach for protein co-purification data reveals high interaction specificity.

Yu X, Ivanic J, Wallqvist A, Reifman J.

PLoS Comput Biol. 2009 Sep;5(9):e1000515. doi: 10.1371/journal.pcbi.1000515. Epub 2009 Sep 25.

PMID:
19779545
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

Analysis of protein-protein interactions using array-based yeast two-hybrid screens.

Rajagopala SV, Uetz P.

Methods Mol Biol. 2009;548:223-45. doi: 10.1007/978-1-59745-540-4_13.

PMID:
19521828
[PubMed - indexed for MEDLINE]
18.

A stringent yeast two-hybrid matrix screening approach for protein-protein interaction discovery.

Worseck JM, Grossmann A, Weimann M, Hegele A, Stelzl U.

Methods Mol Biol. 2012;812:63-87. doi: 10.1007/978-1-61779-455-1_4.

PMID:
22218854
[PubMed - indexed for MEDLINE]
19.

Genome-wide protein interaction maps using two-hybrid systems.

Legrain P, Selig L.

FEBS Lett. 2000 Aug 25;480(1):32-6. Review.

PMID:
10967325
[PubMed - indexed for MEDLINE]
Free Article
20.

High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions.

Chen J, Carter MB, Edwards BS, Cai H, Sklar LA.

Cytometry A. 2012 Jan;81(1):90-8. doi: 10.1002/cyto.a.21144. Epub 2011 Sep 27.

PMID:
21954189
[PubMed - indexed for MEDLINE]
Free PMC Article

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