Format
Items per page
Sort by

Send to:

Choose Destination

Results: 1 to 20 of 104

Similar articles for PubMed (Select 22828411)

1.

A strategy for the expression of recombinant proteins traditionally hard to purify.

Frank EG, McDonald JP, Karata K, Huston D, Woodgate R.

Anal Biochem. 2012 Oct 15;429(2):132-9. doi: 10.1016/j.ab.2012.07.016. Epub 2012 Jul 22.

2.

Type III secretion as a generalizable strategy for the production of full-length biopolymer-forming proteins.

Azam A, Li C, Metcalf KJ, Tullman-Ercek D.

Biotechnol Bioeng. 2015 May 20. doi: 10.1002/bit.25656. [Epub ahead of print]

PMID:
25993982
3.

Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli.

Liu X, Huang A, Luo D, Liu H, Han H, Xu Y, Liang P.

Protein Expr Purif. 2015 May;109:79-84. doi: 10.1016/j.pep.2015.02.010. Epub 2015 Feb 17.

PMID:
25700573
4.

SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with Ulp1.

Kuo D, Nie M, Courey AJ.

Methods Mol Biol. 2014;1177:71-80. doi: 10.1007/978-1-4939-1034-2_6.

PMID:
24943315
5.

High-level expression, purification and characterization of active human C1q and tumour necrosis factor-related protein-1 in Escherichia coli.

Li H, Hui X, Li K, Tang X, Hu X, Xu A, Wu D.

Lett Appl Microbiol. 2014 Sep;59(3):334-41. doi: 10.1111/lam.12280. Epub 2014 Jun 14.

PMID:
24814641
6.

A novel self-cleavage system for production of soluble recombinant protein in Escherichia coli.

Feng Y, Xu Q, Yang T, Sun E, Li J, Shi D, Wu D.

Protein Expr Purif. 2014 Jul;99:64-9. doi: 10.1016/j.pep.2014.04.001. Epub 2014 Apr 13.

PMID:
24727155
7.

Expression and purification of soluble monomeric streptavidin in Escherichia coli.

Demonte D, Dundas CM, Park S.

Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 10.1007/s00253-014-5682-y. Epub 2014 Apr 2.

PMID:
24691867
8.

Expression of soluble and active interferon consensus in SUMO fusion expression system in E. coli.

Peciak K, Tommasi R, Choi JW, Brocchini S, Laurine E.

Protein Expr Purif. 2014 Jul;99:18-26. doi: 10.1016/j.pep.2014.03.009. Epub 2014 Mar 27.

PMID:
24680730
9.

Heterologous expression and purification of dermaseptin S4 fusion in Escherichia coli and recovery of biological activity.

Song D, Chen Y, Li X, Zhu M, Gu Q.

Prep Biochem Biotechnol. 2014;44(6):598-607. doi: 10.1080/10826068.2013.835735.

PMID:
24499364
10.

Codon optimization enhances protein expression of Bombyx mori nucleopolyhedrovirus DNA polymerase in E. coli.

Song H, Li G, Mai W, Huang G, Chen K, Zhou Y, Chen H.

Curr Microbiol. 2014 Mar;68(3):293-300. doi: 10.1007/s00284-013-0476-5. Epub 2013 Oct 16.

PMID:
24129839
11.

[Prokaryotic expression, purification and antigenicity identification of recombinant human survivin protein].

Yin X, Wang W, Tian R, Xu Y, Yan J, Zhang W, Gao J, Yu J.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):877-81. Chinese.

PMID:
23948420
12.

An improved strategy for easy process monitoring and advanced purification of recombinant proteins.

Miladi B, Dridi C, El Marjou A, Boeuf G, Bouallagui H, Dufour F, Di Martino P, Elm'selmi A.

Mol Biotechnol. 2013 Nov;55(3):227-35. doi: 10.1007/s12033-013-9673-5.

PMID:
23780701
13.

Efficient E. coli expression strategies for production of soluble human crystallin ALDH3A1.

Voulgaridou GP, Mantso T, Chlichlia K, Panayiotidis MI, Pappa A.

PLoS One. 2013;8(2):e56582. doi: 10.1371/journal.pone.0056582. Epub 2013 Feb 22.

14.

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.

Scholz J, Besir H, Strasser C, Suppmann S.

BMC Biotechnol. 2013 Feb 14;13:12. doi: 10.1186/1472-6750-13-12.

15.

Generation and functional characterization of the anti-transferrin receptor single-chain antibody-GAL4 (TfRscFv-GAL4) fusion protein.

Ye Q, Hu H, Wang Z, Lu T, Hu Z, Zeng X, Zhang S, Liu J, Lei P, Wang CY, Ye Z, Shen G.

BMC Biotechnol. 2012 Nov 28;12:91. doi: 10.1186/1472-6750-12-91.

16.

Large-scale production of soluble recombinant amyloid-β peptide 1-42 using cold-inducible expression system.

Kim EK, Moon JC, Lee JM, Jeong MS, Oh C, Ahn SM, Yoo YJ, Jang HH.

Protein Expr Purif. 2012 Nov;86(1):53-7. doi: 10.1016/j.pep.2012.08.021. Epub 2012 Sep 13.

PMID:
22982229
17.

Soluble expression and purification of tumor suppressor WT1 and its zinc finger domain.

Fagerlund RD, Ooi PL, Wilbanks SM.

Protein Expr Purif. 2012 Oct;85(2):165-72. doi: 10.1016/j.pep.2012.08.002. Epub 2012 Aug 10.

PMID:
22902878
18.

[Construction of prokaryotic expression vectors for tandem affinity purification].

Wei B, Liao X, Zhou W, Gao Y, Wang Y, Ran J, Liang L, Yue J, Huhe B.

Wei Sheng Wu Xue Bao. 2012 Mar 4;52(3):374-80. Chinese.

PMID:
22712409
19.

Optimized soluble expression and purification of an aggregation-prone protein by fusion tag systems and on-column cleavage in Escherichia coli.

Li W, Gao M, Liu W, Kong Y, Tian H, Yao W, Gao X.

Protein Pept Lett. 2012 Dec;19(12):1324-9.

PMID:
22670677
20.

Orthogonal protein purification facilitated by a small bispecific affinity tag.

Nilvebrant J, Alm T, Hober S.

J Vis Exp. 2012 Jan 16;(59). pii: 3370. doi: 10.3791/3370.

Format
Items per page
Sort by

Send to:

Choose Destination

Supplemental Content

Write to the Help Desk