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Results: 1 to 20 of 106

1.

Using SecM arrest sequence as a tool to isolate ribosome bound polypeptides.

Jha SS, Komar AA.

J Vis Exp. 2012 Jun 19;(64). pii: 4027. doi: 10.3791/4027.

PMID:
22751351
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

Isolation of ribosome bound nascent polypeptides in vitro to identify translational pause sites along mRNA.

Jha SS, Komar AA.

J Vis Exp. 2012 Jul 6;(65). pii: 4026. doi: 10.3791/4026.

PMID:
22806127
[PubMed - indexed for MEDLINE]
Free PMC Article
3.

SsrA tagging of Escherichia coli SecM at its translation arrest sequence.

Collier J, Bohn C, Bouloc P.

J Biol Chem. 2004 Dec 24;279(52):54193-201. Epub 2004 Oct 19.

PMID:
15494397
[PubMed - indexed for MEDLINE]
Free Article
4.

Generation of ribosome nascent chain complexes for structural and functional studies.

Schaffitzel C, Ban N.

J Struct Biol. 2007 Jun;158(3):463-71. Epub 2007 Jan 23.

PMID:
17350284
[PubMed - indexed for MEDLINE]
5.

Large-scale purification of ribosome-nascent chain complexes for biochemical and structural studies.

Rutkowska A, Beerbaum M, Rajagopalan N, Fiaux J, Schmieder P, Kramer G, Oschkinat H, Bukau B.

FEBS Lett. 2009 Jul 21;583(14):2407-13. doi: 10.1016/j.febslet.2009.06.041. Epub 2009 Jun 26.

PMID:
19560460
[PubMed - indexed for MEDLINE]
Free Article
6.

Translation arrest requires two-way communication between a nascent polypeptide and the ribosome.

Woolhead CA, Johnson AE, Bernstein HD.

Mol Cell. 2006 Jun 9;22(5):587-98.

PMID:
16762832
[PubMed - indexed for MEDLINE]
Free Article
7.

SecM-stalled ribosomes adopt an altered geometry at the peptidyl transferase center.

Bhushan S, Hoffmann T, Seidelt B, Frauenfeld J, Mielke T, Berninghausen O, Wilson DN, Beckmann R.

PLoS Biol. 2011 Jan 18;9(1):e1000581. doi: 10.1371/journal.pbio.1000581.

PMID:
21267063
[PubMed - indexed for MEDLINE]
Free PMC Article
8.

Identification of a hyperactive variant of the SecM motif involved in ribosomal arrest.

Ha HJ, Yeom JH, Song WS, Jeon CO, Hahn Y, Lee K.

Curr Microbiol. 2012 Jan;64(1):17-23. doi: 10.1007/s00284-011-0027-x. Epub 2011 Oct 5.

PMID:
21971705
[PubMed - indexed for MEDLINE]
9.

Genetic identification of nascent peptides that induce ribosome stalling.

Tanner DR, Cariello DA, Woolstenhulme CJ, Broadbent MA, Buskirk AR.

J Biol Chem. 2009 Dec 11;284(50):34809-18. doi: 10.1074/jbc.M109.039040. Epub 2009 Oct 19.

PMID:
19840930
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall.

Muto H, Nakatogawa H, Ito K.

Mol Cell. 2006 May 19;22(4):545-52.

PMID:
16713584
[PubMed - indexed for MEDLINE]
Free Article
11.

Prolyl-tRNA(Pro) in the A-site of SecM-arrested ribosomes inhibits the recruitment of transfer-messenger RNA.

Garza-Sánchez F, Janssen BD, Hayes CS.

J Biol Chem. 2006 Nov 10;281(45):34258-68. Epub 2006 Sep 12.

PMID:
16968693
[PubMed - indexed for MEDLINE]
Free PMC Article
12.

The plasticity of a translation arrest motif yields insights into nascent polypeptide recognition inside the ribosome tunnel.

Yap MN, Bernstein HD.

Mol Cell. 2009 Apr 24;34(2):201-11. doi: 10.1016/j.molcel.2009.04.002.

PMID:
19394297
[PubMed - indexed for MEDLINE]
Free PMC Article
13.

Nascent peptides that block protein synthesis in bacteria.

Woolstenhulme CJ, Parajuli S, Healey DW, Valverde DP, Petersen EN, Starosta AL, Guydosh NR, Johnson WE, Wilson DN, Buskirk AR.

Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):E878-87. doi: 10.1073/pnas.1219536110. Epub 2013 Feb 19.

PMID:
23431150
[PubMed - indexed for MEDLINE]
Free PMC Article
14.

Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.

Shirole N, Balasubramanian S, Yanofsky C, Cruz-Vera L.

J Vis Exp. 2011 Feb 25;(48). pii: 2498. doi: 10.3791/2498.

PMID:
21403627
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability.

Contreras-Martínez LM, DeLisa MP.

J Mol Biol. 2007 Sep 14;372(2):513-24. Epub 2007 Jul 3.

PMID:
17669427
[PubMed - indexed for MEDLINE]
16.

Translation arrest of SecM is essential for the basal and regulated expression of SecA.

Murakami A, Nakatogawa H, Ito K.

Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12330-5. Epub 2004 Aug 9.

PMID:
15302932
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

Elongation and folding of nascent ricin chains as peptidyl-tRNA on ribosomes: the effect of amino acid deletions on these processes.

Kudlicki W, Kitaoka Y, Odom OW, Kramer G, Hardesty B.

J Mol Biol. 1995 Sep 15;252(2):203-12.

PMID:
7674301
[PubMed - indexed for MEDLINE]
18.

Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements.

Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J.

Mol Cell. 2006 May 19;22(4):533-43.

PMID:
16713583
[PubMed - indexed for MEDLINE]
Free Article
19.

Mechanisms of SecM-mediated stalling in the ribosome.

Gumbart J, Schreiner E, Wilson DN, Beckmann R, Schulten K.

Biophys J. 2012 Jul 18;103(2):331-41. doi: 10.1016/j.bpj.2012.06.005. Epub 2012 Jul 17.

PMID:
22853911
[PubMed - indexed for MEDLINE]
Free PMC Article
20.

Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia coli.

Sunohara T, Jojima K, Tagami H, Inada T, Aiba H.

J Biol Chem. 2004 Apr 9;279(15):15368-75. Epub 2004 Jan 26.

PMID:
14744860
[PubMed - indexed for MEDLINE]
Free Article

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