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Results: 1 to 20 of 163

Similar articles for PubMed (Select 22706479)

1.

Assessment of immobilized PGA orientation via the LC-MS analysis of tryptic digests of the wild type and its 3K-PGA mutant assists in the rational design of a high-performance biocatalyst.

Serra I, Ubiali D, Cecchini DA, Calleri E, Albertini AM, Terreni M, Temporini C.

Anal Bioanal Chem. 2013 Jan;405(2-3):745-53. doi: 10.1007/s00216-012-6143-z. Epub 2012 Jun 19.

PMID:
22706479
2.

Characterization and study of the orientation of immobilized enzymes by tryptic digestion and HPLC-MS: design of an efficient catalyst for the synthesis of cephalosporins.

Temporini C, Bonomi P, Serra I, Tagliani A, Bavaro T, Ubiali D, Massolini G, Terreni M.

Biomacromolecules. 2010 Jun 14;11(6):1623-32. doi: 10.1021/bm100259a.

PMID:
20462204
3.

New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase.

Cecchini DA, Serra I, Ubiali D, Terreni M, Albertini AM.

BMC Biotechnol. 2007 Sep 10;7:54.

4.

Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase.

Cecchini DA, Pavesi R, Sanna S, Daly S, Xaiz R, Pregnolato M, Terreni M.

Appl Microbiol Biotechnol. 2012 Sep;95(6):1491-500. doi: 10.1007/s00253-011-3817-y. Epub 2012 Jan 8.

PMID:
22228258
5.

Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers.

Montes T, Grazú V, López-Gallego F, Hermoso JA, García JL, Manso I, Galán B, González R, Fernández-Lafuente R, Guisán JM.

Appl Environ Microbiol. 2007 Jan;73(1):312-9. Epub 2006 Nov 10.

6.

Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C.

Terreni M, Pagani G, Ubiali D, Fernández-Lafuente R, Mateo C, Guisán JM.

Bioorg Med Chem Lett. 2001 Sep 17;11(18):2429-32.

PMID:
11549439
7.

Improving the diastereoselectivity of penicillin G acylase for ampicillin synthesis from racemic substrates.

Deaguero AL, Blum JK, Bommarius AS.

Protein Eng Des Sel. 2012 Mar;25(3):135-44. doi: 10.1093/protein/gzr065. Epub 2012 Jan 22.

8.

Modulation of the microenvironment surrounding the active site of penicillin G acylase immobilized on acrylic carriers improves the enzymatic synthesis of cephalosporins.

Bonomi P, Bavaro T, Serra I, Tagliani A, Terreni M, Ubiali D.

Molecules. 2013 Nov 20;18(11):14349-65. doi: 10.3390/molecules181114349.

9.

A new biocatalyst: Penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties.

Bernardino SM, Fernandes P, Fonseca LP.

Biotechnol J. 2009 May;4(5):695-702. doi: 10.1002/biot.200800287.

PMID:
19418472
10.

Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag.

Scaramozzino F, Estruch I, Rossolillo P, Terreni M, Albertini AM.

Appl Environ Microbiol. 2005 Dec;71(12):8937-40.

11.

Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment.

Abian O, Grazú V, Hermoso J, González R, García JL, Fernández-Lafuente R, Guisán JM.

Appl Environ Microbiol. 2004 Feb;70(2):1249-51.

12.

Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins.

Estruch I, Tagliani AR, Guisán JM, Fernández-Lafuente R, Alcántara AR, Toma L, Terreni M.

Enzyme Microb Technol. 2008 Jan;42(2):121-9. doi: 10.1016/j.enzmictec.2007.08.013. Epub 2007 Sep 6.

PMID:
22578861
13.

Hydrogel coated monoliths for enzymatic hydrolysis of penicillin G.

de Lathouder KM, Smeltink MW, Straathof AJ, Paasman MA, van de Sandt EJ, Kapteijn F, Moulijn JA.

J Ind Microbiol Biotechnol. 2008 Aug;35(8):815-24. doi: 10.1007/s10295-008-0353-6. Epub 2008 Apr 22.

14.

Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues.

Oh B, Kim K, Park J, Yoon J, Han D, Kim Y.

Biochem Biophys Res Commun. 2004 Jun 25;319(2):486-92.

PMID:
15178432
15.

Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies.

Alkema WB, Hensgens CM, Kroezinga EH, de Vries E, Floris R, van der Laan JM, Dijkstra BW, Janssen DB.

Protein Eng. 2000 Dec;13(12):857-63.

16.

Glyoxyl-disulfide agarose: a tailor-made support for site-directed rigidification of proteins.

Godoy CA, de las Rivas B, Grazú V, Montes T, Guisán JM, López-Gallego F.

Biomacromolecules. 2011 May 9;12(5):1800-9. doi: 10.1021/bm200161f. Epub 2011 Apr 8.

PMID:
21413783
17.

Immobilized penicillin G acylase as reactor and chiral selector in liquid chromatography.

Massolini G, Calleri E, De Lorenzi E, Pregnolato M, Terreni M, Félix G, Gandini C.

J Chromatogr A. 2001 Jul 6;921(2):147-60.

PMID:
11471798
18.

Role of alphaArg145 and betaArg263 in the active site of penicillin acylase of Escherichia coli.

Alkema WB, Prins AK, de Vries E, Janssen DB.

Biochem J. 2002 Jul 1;365(Pt 1):303-9.

19.

Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis.

Gabor EM, Janssen DB.

Protein Eng Des Sel. 2004 Jul;17(7):571-9. Epub 2004 Aug 27.

20.

Immobilization of penicillin G acylase on paramagnetic aldehyde-functionalized mesostructured cellular foams.

Yang L, Gao Z, Guo Y, Zhan W, Guo Y, Wang Y, Lu G.

Enzyme Microb Technol. 2014 Jun 10;60:32-9. doi: 10.1016/j.enzmictec.2014.03.011. Epub 2014 Apr 4.

PMID:
24835097
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