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Results: 1 to 20 of 88

1.

Optimization of purification protocols based on the step-by-step monitoring of the protein aggregates in soluble fractions.

de Marco A.

Methods Mol Biol. 2012;824:145-54. doi: 10.1007/978-1-61779-433-9_7.

PMID:
22160897
[PubMed - indexed for MEDLINE]
2.

Strategies for boosting the accumulation of correctly folded recombinant proteins expressed in Escherichia coli.

de Marco A.

Methods Mol Biol. 2011;752:1-15. doi: 10.1007/978-1-60327-223-0_1.

PMID:
21713627
[PubMed - indexed for MEDLINE]
3.

Screening optimized protein purification protocols by coupling small-scale expression and mini-size exclusion chromatography.

Sala E, de Marco A.

Protein Expr Purif. 2010 Dec;74(2):231-5. doi: 10.1016/j.pep.2010.05.014. Epub 2010 May 31.

PMID:
20685308
[PubMed - indexed for MEDLINE]
4.

Screening methods to determine biophysical properties of proteins in structural genomics.

Woestenenk EA, Hammarström M, Härd T, Berglund H.

Anal Biochem. 2003 Jul 1;318(1):71-9.

PMID:
12782033
[PubMed - indexed for MEDLINE]
5.

A strategy for optimizing the monodispersity of fusion proteins: application to purification of recombinant HPV E6 oncoprotein.

Nominé Y, Ristriani T, Laurent C, Lefèvre JF, Weiss E, Travé G.

Protein Eng. 2001 Apr;14(4):297-305.

PMID:
11391022
[PubMed - indexed for MEDLINE]
Free Article
6.
7.

In vivo and in vitro protein solubility assays using split GFP.

Cabantous S, Waldo GS.

Nat Methods. 2006 Oct;3(10):845-54.

PMID:
16990817
[PubMed - indexed for MEDLINE]
8.

Induced fit of passenger proteins fused to Archaea maltose binding proteins.

Huang H, Liu J, de Marco A.

Biochem Biophys Res Commun. 2006 May 26;344(1):25-9. Epub 2006 Mar 31.

PMID:
16615999
[PubMed - indexed for MEDLINE]
9.

Purification of gamma-crystallin from human lenses by acetone precipitation method.

Srivastava OP, Srivastava K.

Curr Eye Res. 1998 Nov;17(11):1074-81.

PMID:
9846626
[PubMed - indexed for MEDLINE]
10.

Folding and purification of insoluble (inclusion body) proteins from Escherichia coli.

Wingfield PT, Palmer I, Liang SM.

Curr Protoc Protein Sci. 2001 May;Chapter 6:Unit 6.5. doi: 10.1002/0471140864.ps0605s00.

PMID:
18429192
[PubMed - indexed for MEDLINE]
11.

Purification of the Caenorhabditis elegans transposase Tc1A refolded during gel filtration chromatography.

García-Sáez I, Plasterk RH.

Protein Expr Purif. 2000 Aug;19(3):355-61.

PMID:
10910725
[PubMed - indexed for MEDLINE]
12.

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.

Cabrita LD, Dai W, Bottomley SP.

BMC Biotechnol. 2006 Mar 1;6:12.

PMID:
16509985
[PubMed - indexed for MEDLINE]
Free PMC Article
13.

High-throughput protein purification under denaturating conditions by the use of cation exchange chromatography.

Alm T, Steen J, Ottosson J, Hober S.

Biotechnol J. 2007 Jun;2(6):709-16.

PMID:
17492715
[PubMed - indexed for MEDLINE]
14.

Parallel production and verification of protein products using a novel high-throughput screening method.

Tegel H, Yderland L, Boström T, Eriksson C, Ukkonen K, Vasala A, Neubauer P, Ottosson J, Hober S.

Biotechnol J. 2011 Aug;6(8):1018-25. doi: 10.1002/biot.201000430. Epub 2011 Jun 16.

PMID:
21681961
[PubMed - indexed for MEDLINE]
15.

Design of protein purification pathways: application to the proteome of Haemophilus influenzae using heparin chromatography.

Fountoulakis M, Takács B.

Protein Expr Purif. 1998 Oct;14(1):113-9.

PMID:
9758758
[PubMed - indexed for MEDLINE]
16.

Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis.

Scheich C, Leitner D, Sievert V, Leidert M, Schlegel B, Simon B, Letunic I, Büssow K, Diehl A.

BMC Struct Biol. 2004 Mar 8;4:4.

PMID:
15113422
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

Quantitation of soluble aggregates in recombinant monoclonal antibody cell culture by pH-gradient protein A chromatography.

Pan H, Chen K, Pulisic M, Apostol I, Huang G.

Anal Biochem. 2009 May 15;388(2):273-8. doi: 10.1016/j.ab.2009.02.037. Epub 2009 Mar 4.

PMID:
19268420
[PubMed - indexed for MEDLINE]
18.

High-throughput protein expression screening and purification in Escherichia coli.

Vincentelli R, Cimino A, Geerlof A, Kubo A, Satou Y, Cambillau C.

Methods. 2011 Sep;55(1):65-72. doi: 10.1016/j.ymeth.2011.08.010. Epub 2011 Sep 8.

PMID:
21925268
[PubMed - indexed for MEDLINE]
19.

Recombinant production of bioactive human TNF-alpha by SUMO-fusion system--high yields from shake-flask culture.

Hoffmann A, Müller MQ, Gloser M, Sinz A, Rudolph R, Pfeifer S.

Protein Expr Purif. 2010 Aug;72(2):238-43. doi: 10.1016/j.pep.2010.03.022. Epub 2010 Apr 2.

PMID:
20363332
[PubMed - indexed for MEDLINE]
20.

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