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Items: 1 to 20 of 98

1.

PCR Mutagenesis by Overlap Extension and Gene SOE.

Vallejo AN, Pogulis RJ, Pease LR.

CSH Protoc. 2008 Feb 1;2008:pdb.prot4861. doi: 10.1101/pdb.prot4861.

PMID:
21356760
2.

Gene splicing and mutagenesis by PCR-driven overlap extension.

Heckman KL, Pease LR.

Nat Protoc. 2007;2(4):924-32.

PMID:
17446874
4.

Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR.

Gene. 1989 Apr 15;77(1):51-9.

PMID:
2744487
6.

DNA splicing by directed ligation (SDL).

Berlin YA.

Curr Issues Mol Biol. 1999;1(1-2):21-30.

7.

[Polymerase chain reaction, cold probes and clinical diagnosis].

Haras D, Amoros JP.

Sante. 1994 Jan-Feb;4(1):43-52. Review. French.

PMID:
7909267
8.

Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.

Horton RM, Cai ZL, Ho SN, Pease LR.

Biotechniques. 1990 May;8(5):528-35.

PMID:
2357375
9.

A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase.

Rashtchian A, Thornton CG, Heidecker G.

PCR Methods Appl. 1992 Nov;2(2):124-30.

12.

A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR.

An Y, Ji J, Wu W, Lv A, Huang R, Wei Y.

Appl Microbiol Biotechnol. 2005 Oct;68(6):774-8. Epub 2005 Oct 13.

PMID:
16249879
13.

Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension.

Kumar R, Rajagopal K.

Anal Biochem. 2008 Jun 1;377(1):105-7. doi: 10.1016/j.ab.2008.03.003. Epub 2008 Mar 7.

PMID:
18371292
14.

Insertion and deletion mutagenesis by overlap extension PCR.

Lee J, Shin MK, Ryu DK, Kim S, Ryu WS.

Methods Mol Biol. 2010;634:137-46. doi: 10.1007/978-1-60761-652-8_10.

PMID:
20676981
15.

Sticky PCR: A PCR-based protocol for targeted protein engineering.

Yamabhai M.

Biotechnol J. 2009 Apr;4(4):544-53. doi: 10.1002/biot.200800198.

PMID:
19370716
16.

PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

Kuwayama H, Obara S, Morio T, Katoh M, Urushihara H, Tanaka Y.

Nucleic Acids Res. 2002 Jan 15;30(2):E2.

17.

Long RT-PCR of the entire 8.5-kb NF1 open reading frame and mutation detection on agarose gels.

Martinez JM, Breidenbach HH, Cawthon R.

Genome Res. 1996 Jan;6(1):58-66.

18.
19.

Creating seamless junctions independent of restriction sites in PCR cloning.

Padgett KA, Sorge JA.

Gene. 1996 Feb 2;168(1):31-5.

PMID:
8626061
20.

Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags.

Antal Z, Rascle C, Fèvre M, Bruel C.

Curr Genet. 2004 Oct;46(4):240-6. Epub 2004 Sep 2.

PMID:
15349749
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