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Genome-wide fitness and genetic interactions determined by Tn-seq, a high-throughput massively parallel sequencing method for microorganisms.

van Opijnen T, Camilli A.

Curr Protoc Microbiol. 2010 Nov;Chapter 1:Unit1E.3. doi: 10.1002/9780471729259.mc01e03s19.


Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms.

van Opijnen T, Lazinski DW, Camilli A.

Curr Protoc Mol Biol. 2014 Apr 14;106:7.16.1-7.16.24. doi: 10.1002/0471142727.mb0716s106.


Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms.

van Opijnen T, Lazinski DW, Camilli A.

Curr Protoc Microbiol. 2015 Feb 2;36:1E.3.1-1E.3.24. doi: 10.1002/9780471729259.mc01e03s36.


Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms.

van Opijnen T, Bodi KL, Camilli A.

Nat Methods. 2009 Oct;6(10):767-72. doi: 10.1038/nmeth.1377. Epub 2009 Sep 20.


Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms.

van Opijnen T, Camilli A.

Nat Rev Microbiol. 2013 Jul;11(7):435-42. doi: 10.1038/nrmicro3033. Epub 2013 May 28.


Rapid transposon liquid enrichment sequencing (TnLE-seq) for gene fitness evaluation in underdeveloped bacterial systems.

Fels SR, Zane GM, Blake SM, Wall JD.

Appl Environ Microbiol. 2013 Dec;79(23):7510-7. doi: 10.1128/AEM.02051-13. Epub 2013 Sep 27.


Genome scanning for conditionally essential genes in Salmonella enterica Serotype Typhimurium.

Khatiwara A, Jiang T, Sung SS, Dawoud T, Kim JN, Bhattacharya D, Kim HB, Ricke SC, Kwon YM.

Appl Environ Microbiol. 2012 May;78(9):3098-107. doi: 10.1128/AEM.06865-11. Epub 2012 Feb 24.


Development of genomic array footprinting for identification of conditionally essential genes in Streptococcus pneumoniae.

Bijlsma JJ, Burghout P, Kloosterman TG, Bootsma HJ, de Jong A, Hermans PW, Kuipers OP.

Appl Environ Microbiol. 2007 Mar;73(5):1514-24. Epub 2007 Jan 19.


Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq.

Gallagher LA, Shendure J, Manoil C.

MBio. 2011 Jan 18;2(1):e00315-10. doi: 10.1128/mBio.00315-10.


A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon libraries.

Skurnik D, Roux D, Aschard H, Cattoir V, Yoder-Himes D, Lory S, Pier GB.

PLoS Pathog. 2013;9(9):e1003582. doi: 10.1371/journal.ppat.1003582. Epub 2013 Sep 5.


Approaches to querying bacterial genomes with transposon-insertion sequencing.

Barquist L, Boinett CJ, Cain AK.

RNA Biol. 2013 Jul;10(7):1161-9. doi: 10.4161/rna.24765. Epub 2013 Apr 23. Review.


ESSENTIALS: software for rapid analysis of high throughput transposon insertion sequencing data.

Zomer A, Burghout P, Bootsma HJ, Hermans PW, van Hijum SA.

PLoS One. 2012;7(8):e43012. doi: 10.1371/journal.pone.0043012. Epub 2012 Aug 10.


Rapid 96-well plates DNA extraction and sequencing procedures to identify genome-wide transposon insertion sites in a difficult to lyse bacterium: Lactobacillus casei.

Scornec H, Tichit M, Bouchier C, P├ędron T, Cavin JF, Sansonetti PJ, Licandro-Seraut H.

J Microbiol Methods. 2014 Nov;106:78-82. doi: 10.1016/j.mimet.2014.08.001. Epub 2014 Aug 16.


High-throughput insertion tracking by deep sequencing for the analysis of bacterial pathogens.

Wong SM, Gawronski JD, Lapointe D, Akerley BJ.

Methods Mol Biol. 2011;733:209-22. doi: 10.1007/978-1-61779-089-8_15.


A fine scale phenotype-genotype virulence map of a bacterial pathogen.

van Opijnen T, Camilli A.

Genome Res. 2012 Dec;22(12):2541-51. doi: 10.1101/gr.137430.112. Epub 2012 Jul 23.


Calling Cards enable multiplexed identification of the genomic targets of DNA-binding proteins.

Wang H, Mayhew D, Chen X, Johnston M, Mitra RD.

Genome Res. 2011 May;21(5):748-55. doi: 10.1101/gr.114850.110. Epub 2011 Apr 6.


A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology.

Morozumi T, Toki D, Eguchi-Ogawa T, Uenishi H.

Biotechniques. 2011 Sep;51(3):195-7. doi: 10.2144/000113737.


Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq.

Brutinel ED, Gralnick JA.

Mol Microbiol. 2012 Oct;86(2):273-83. doi: 10.1111/j.1365-2958.2012.08196.x. Epub 2012 Aug 27.


Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions.

Deutschbauer A, Price MN, Wetmore KM, Shao W, Baumohl JK, Xu Z, Nguyen M, Tamse R, Davis RW, Arkin AP.

PLoS Genet. 2011 Nov;7(11):e1002385. doi: 10.1371/journal.pgen.1002385. Epub 2011 Nov 17.

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