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Identification of mutations in a candidate dengue 4 vaccine strain 341750 PDK20 and construction of a full-length cDNA clone of the PDK20 vaccine candidate.

Kelly EP, Puri B, Sun W, Falgout B.

Vaccine. 2010 Apr 9;28(17):3030-7. doi: 10.1016/j.vaccine.2009.10.084. Epub 2009 Oct 27.


Evolution of attenuating mutations in dengue-2 strain S16803 PDK50 vaccine and comparison of growth kinetics with parent virus.

Kelly EP, Polo S, Sun W, Falgout B.

Virus Genes. 2011 Aug;43(1):18-26. doi: 10.1007/s11262-011-0602-z. Epub 2011 Apr 3.


Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53.

Kinney RM, Butrapet S, Chang GJ, Tsuchiya KR, Roehrig JT, Bhamarapravati N, Gubler DJ.

Virology. 1997 Apr 14;230(2):300-8.


Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells.

Puri B, Nelson WM, Henchal EA, Hoke CH, Eckels KH, Dubois DR, Porter KR, Hayes CG.

J Gen Virol. 1997 Sep;78 ( Pt 9):2287-91.


Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys.

Eckels KH, Dubois DR, Putnak R, Vaughn DW, Innis BL, Henchal EA, Hoke CH Jr.

Am J Trop Med Hyg. 2003 Dec;69(6 Suppl):12-6. Erratum in: Am J Trop Med Hyg. 2004 Mar;70(3):336.


Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. I. Attributes of uncloned virus at different passage levels.

Halstead SB, Diwan AR, Marchette NJ, Palumbo NE, Srisukonth L.

Am J Trop Med Hyg. 1984 Jul;33(4):654-65.


Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. IV. Characterization of a vaccine candidate in fetal rhesus lung cells.

Halstead SB, Eckels KH, Putvatana R, Larsen LK, Marchette NJ.

Am J Trop Med Hyg. 1984 Jul;33(4):679-83.


A live attenuated dengue-1 vaccine candidate (45AZ5) passaged in primary dog kidney cell culture is attenuated and immunogenic for humans.

Edelman R, Tacket CO, Wasserman SS, Vaughn DW, Eckels KH, Dubois DR, Summers PL, Hoke CH.

J Infect Dis. 1994 Dec;170(6):1448-55.


A single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys.

Guirakhoo F, Zhang Z, Myers G, Johnson BW, Pugachev K, Nichols R, Brown N, Levenbook I, Draper K, Cyrek S, Lang J, Fournier C, Barrere B, Delagrave S, Monath TP.

J Virol. 2004 Sep;78(18):9998-10008.


Preparation of an attenuated dengue 4 (341750 Carib) virus vaccine. I. Pre-clinical studies.

Marchette NJ, Dubois DR, Larsen LK, Summers PL, Kraiselburd EG, Gubler DJ, Eckels KH.

Am J Trop Med Hyg. 1990 Aug;43(2):212-8.


Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2.

Blaney JE Jr, Hanson CT, Hanley KA, Murphy BR, Whitehead SS.

BMC Infect Dis. 2004 Oct 4;4:39.


Dengue type 4 live-attenuated vaccine viruses passaged in vero cells affect genetic stability and dengue-induced hemorrhaging in mice.

Lee HC, Yen YT, Chen WY, Wu-Hsieh BA, Wu SC.

PLoS One. 2011;6(10):e25800. doi: 10.1371/journal.pone.0025800. Epub 2011 Oct 28.


West Nile virus/dengue type 4 virus chimeras that are reduced in neurovirulence and peripheral virulence without loss of immunogenicity or protective efficacy.

Pletnev AG, Putnak R, Speicher J, Wagar EJ, Vaughn DW.

Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3036-41. Erratum in: Proc Natl Acad Sci U S A 2002 May 14;99(10):7184.


Replication of the primary dog kidney-53 dengue 2 virus vaccine candidate in Aedes aegypti is modulated by a mutation in the 5' untranslated region and amino acid substitutions in nonstructural proteins 1 and 3.

Brault AC, Kinney RM, Maharaj PD, Green EN, Reisen WK, Huang CY.

Vector Borne Zoonotic Dis. 2011 Jun;11(6):683-9. doi: 10.1089/vbz.2010.0150. Epub 2011 Feb 1.

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