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Items: 1 to 20 of 88

1.

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.

Ruijter JM, Ramakers C, Hoogaars WM, Karlen Y, Bakker O, van den Hoff MJ, Moorman AF.

Nucleic Acids Res. 2009 Apr;37(6):e45. doi: 10.1093/nar/gkp045. Epub 2009 Feb 22.

2.

Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process.

Smith MV, Miller CR, Kohn M, Walker NJ, Portier CJ.

BMC Bioinformatics. 2007 Oct 23;8:409.

3.

Stepwise kinetic equilibrium models of quantitative polymerase chain reaction.

Cobbs G.

BMC Bioinformatics. 2012 Aug 16;13:203. doi: 10.1186/1471-2105-13-203.

4.
5.

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry.

Spiess AN, Feig C, Ritz C.

BMC Bioinformatics. 2008 Apr 29;9:221. doi: 10.1186/1471-2105-9-221.

6.

Comprehensive algorithm for quantitative real-time polymerase chain reaction.

Zhao S, Fernald RD.

J Comput Biol. 2005 Oct;12(8):1047-64.

7.

Statistical algorithm for assuring similar efficiency in standards and samples for absolute quantification by real-time reverse transcription polymerase chain reaction.

Chervoneva I, Hyslop T, Iglewicz B, Johns L, Wolfe HR, Schulz S, Leong E, Waldman S.

Anal Biochem. 2006 Jan 15;348(2):198-208. Epub 2005 Nov 17.

PMID:
16336939
8.

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.

Ramakers C, Ruijter JM, Deprez RH, Moorman AF.

Neurosci Lett. 2003 Mar 13;339(1):62-6.

PMID:
12618301
9.

Statistical tools for transgene copy number estimation based on real-time PCR.

Yuan JS, Burris J, Stewart NR, Mentewab A, Stewart CN Jr.

BMC Bioinformatics. 2007 Nov 1;8 Suppl 7:S6.

10.

Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value.

Tuomi JM, Voorbraak F, Jones DL, Ruijter JM.

Methods. 2010 Apr;50(4):313-22. doi: 10.1016/j.ymeth.2010.02.003. Epub 2010 Feb 6.

PMID:
20138998
11.

Statistical methods for efficiency adjusted real-time PCR quantification.

Yuan JS, Wang D, Stewart CN Jr.

Biotechnol J. 2008 Jan;3(1):112-23.

PMID:
18074404
12.

[Use of the real-time RT-PCR method for investigation of small stable RNA expression level in human epidermoid carcinoma cells A431].

Nikitina TV, Nazarova NIu, Tishchenko LI, Tuohimaa P, Sedova VM.

Tsitologiia. 2003;45(4):392-402. Russian.

PMID:
14520871
13.
14.

Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence.

Batsch A, Noetel A, Fork C, Urban A, Lazic D, Lucas T, Pietsch J, Lazar A, Sch√∂mig E, Gr√ľndemann D.

BMC Bioinformatics. 2008 Feb 12;9:95. doi: 10.1186/1471-2105-9-95.

15.

Progress curve analysis of qRT-PCR reactions using the logistic growth equation.

Liu M, Udhe-Stone C, Goudar CT.

Biotechnol Prog. 2011 Sep-Oct;27(5):1407-14. doi: 10.1002/btpr.666. Epub 2011 Jul 15.

PMID:
21766473
16.

Real-time PCR quantification using a variable reaction efficiency model.

Platts AE, Johnson GD, Linnemann AK, Krawetz SA.

Anal Biochem. 2008 Sep 15;380(2):315-22. doi: 10.1016/j.ab.2008.05.048. Epub 2008 Jun 5.

17.

Error minimization algorithm for comparative quantitative PCR analysis: Q-Anal.

OConnor W, Runquist EA.

Anal Biochem. 2008 Jul 1;378(1):96-8. doi: 10.1016/j.ab.2008.03.041. Epub 2008 Mar 28.

PMID:
18420021
18.

Quantification of mRNA in single oocytes and embryos by real-time rapid cycle fluorescence monitored RT-PCR.

Steuerwald N, Cohen J, Herrera RJ, Brenner CA.

Mol Hum Reprod. 2000 May;6(5):448-53.

19.

A Java program for LRE-based real-time qPCR that enables large-scale absolute quantification.

Rutledge RG.

PLoS One. 2011 Mar 2;6(3):e17636. doi: 10.1371/journal.pone.0017636.

20.

Standardized determination of real-time PCR efficiency from a single reaction set-up.

Tichopad A, Dilger M, Schwarz G, Pfaffl MW.

Nucleic Acids Res. 2003 Oct 15;31(20):e122. Erratum in: Nucleic Acids Res. 2003 Nov 15;31(22):6688.

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