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18583086,21865001,19773088,16207951,25233940,17553740,15994048,19011729,33167379,11682539,15332281,21906409,27098955,17609830,15664052,18597860,15163417,25346397,19628911,18063164,18814485,18652847,15583346,16597854,20827768,16930266,22670036,8313184,14695431,19815886,20434124,19842428,19748424,18551599,29751761,19345140,15115087,17215345,16628591,12083330,16898316,22099888,12116030,15722024,20981793,17135444,21399603,22299407,29979812,18815724,17827371,19822173,18951921,15921589,31557664,24889214,25455901,21341545,9607089,18248565,11130884,12822553,17290754,23896021,18360905,29382300,11556585,22301475,17642533,16883281,15780804,17573132,17409201,28254680,18501437,20116813,26113247,19323010,29016291,20670467,9139373,20578057,26455921,15691143,23669102,15640856,17075660,7855637,26247719,20578459,18503610,23850695,15906664,22898479,9805040,15639750,15916043,29063884,27731556,25183027,17522268,12118459,18639489,16110945,18406488,31088375,15916091,20578487,30268947,15041181,16524419,19166574,15941596,23313361,18503611,22469818,16527238,19642386,21116795,15251405,21874746,15617345,25568438,16632907,20600330,21323170,17229857,11719982,18840736,12971495,32157991,24132555,29643334,20848853,16962821,11473994,18592845,15737419,27477452,16880757,17911000,27030492,12526321,11414441,19725926,17403544,30029808,27479173,11911593,14524480,26527283,26509163,21798263,15516646,29761765,17061532,31376506,12820947,15163416,20966558,19042152,22274167,10555455,22766181,9887321,28910810,17627877,18631309,26991052,22632412,20957194,16624755,24650864,17038699,16083176,17140671,23532380,17333763,31388071,18234069,22546647,15956414,12791857,18778736,11269364,19861623,30563591,19261315,18974508,17186958,18495513,23781709,19725384,18602422,18400327,16824209,16821435,19463715,22964720,27510671,16898330,29436418,16096709,29363451,17492597,11574589,25219286,16982455,19500619,19024422,27184629,21371218,19591873,11436695,17583360,22670858,10479118,20563439,20942072,15847925,17356302,14556266,26572227,23964963,27614319,26416109,19442836,18165535,28427352,27393681,27277216,18951923,18940268,17317885,17207498,19428586,26375910,19050355,15961136,18945836,16019259,19442576,18790707,12665913,17034872,27905632,21410643,18187788,19559729,17584525,19539655,19185597,12828344,15619909,15683998,12174774,19523981,25600599,28468604,16221971,19168965,18541782,18611411,15691147,23637298,18387217,16222028,16438144,21437996,19683467,18761376,27774649,19061754,17920211,17000012,17964668,17065431,7738139,18313146,19624923,18384900,23563693,19501535,18972604,19122918,19058574,8474356,23806706,19010450,18205212,25145064,10713370,30291875,18692099,18685000,25420667,18631262,30680559,23875046,19059437,15672450,29513820,18551940,22302871,27524929,30994095,18434933,11377710,20054667,17869351,35536436,11944700,9705406,30084423,29813062,17700030,24652263,25766980,20614726,9158052,25522325,27656926,25453334,15619908,30180800,29813065,12751316,29611509,18982804,21760476,17586592,26032430,27172387,16212996,32843276,35746742,26075598,17881871,18219146,17996971,17617845,18219080,21689012,18219129,31202830,18400240,25533614,30451226,11073786,12886958,21803032,17122650,23886793,14711604,25102066,20881182,9766894,9130232,23082554,22776389,10970375,1372617,17686535,18217869,29607357,23821270,27233265,17475342,15603717,10586901,17172379,17416433,18320112,9394519,30272614,24776933,26580561,30610766,9566225,18243816,20105295,17541699,27571201,18191569,18280588,17156880,17905372,1951850,31443243,12757228,17418320,5827198,18191361,9332607,22825831,19483079,22502614,21265245,23384180,21843553,28748843,19625474,10674684,18382348,31932713,18288627,23219893,25056332,27622791,16978853,18397467,20550431,10674666,12454138,26794427,11857532,20092632,16971176,5301572,9522986,17006599,24159550,17457917,28317856,32134380,33601777,18499276,17376878,27334982,14500123,3022710,29514934,32669639,31124226,27139219,690610,30498319,20957606,24453838,4388910,26230127,12363071,12904404,9830728,17959198,15488624,11172984,17134132,28954066,23862412,17984351,25373042,25504239,18486073,5832545,16316157,34709641,19239767,18567444,6038994,18028362,24367579,18315529,5984948,18181502,26872358,25461177,1713265,18373533,32932665,34940236,16785712,28444295,6118053,18336923,24744402,24744403,26010246,12958495,18651905,31933466,34174430,32792638,12170337,26851784,1885943,27455967,25728040,11777856,7464570,10385025,25418184,26017916,18215633,35693657,17297040,17854768,26562299,6323109,26246086,16874176,5930492,18519071,17490597,17698025,26407434,24479040,30876573,11476552,25248522,18387678,6298956,18312184,25195216,15136178,27365916,15645983,27134872,10694165,2156139,3018470,31260132,3026069,3240934,13556870,2994265,16533009[uid]

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Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection.

Dash PK, Parida M, Santhosh SR, Saxena P, Srivastava A, Neeraja M, Lakshmi V, Rao PV. Dash PK, et al. Diagn Microbiol Infect Dis. 2008 Sep;62(1):52-7. doi: 10.1016/j.diagmicrobio.2008.05.002. Epub 2008 Jun 25. Diagn Microbiol Infect Dis. 2008. PMID: 18583086

Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples.

Mishra B, Sharma M, Pujhari SK, Ratho RK, Gopal DS, Kumar CN, Sarangi G, Chayani N, Varma SC. Mishra B, et al. Diagn Microbiol Infect Dis. 2011 Oct;71(2):118-25. doi: 10.1016/j.diagmicrobio.2011.06.020. Epub 2011 Aug 23. Diagn Microbiol Infect Dis. 2011. PMID: 21865001

Simultaneous detection and quantitation of Chikungunya, dengue and West Nile viruses by multiplex RT-PCR assays and dengue virus typing using high resolution melting.

Naze F, Le Roux K, Schuffenecker I, Zeller H, Staikowsky F, Grivard P, Michault A, Laurent P. Naze F, et al. J Virol Methods. 2009 Dec;162(1-2):1-7. doi: 10.1016/j.jviromet.2009.03.006. Epub 2009 Mar 17. J Virol Methods. 2009. PMID: 19773088

Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay.

Johnson BW, Russell BJ, Lanciotti RS. Johnson BW, et al. J Clin Microbiol. 2005 Oct;43(10):4977-83. doi: 10.1128/JCM.43.10.4977-4983.2005. J Clin Microbiol. 2005. PMID: 16207951 Free PMC article.

Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses.

Cecilia D, Kakade M, Alagarasu K, Patil J, Salunke A, Parashar D, Shah PS. Cecilia D, et al. Arch Virol. 2015 Jan;160(1):323-7. doi: 10.1007/s00705-014-2217-x. Epub 2014 Sep 19. Arch Virol. 2015. PMID: 25233940

Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus.

Santhosh SR, Parida MM, Dash PK, Pateriya A, Pattnaik B, Pradhan HK, Tripathi NK, Ambuj S, Gupta N, Saxena P, Lakshmana Rao PV. Santhosh SR, et al. J Clin Virol. 2007 Jul;39(3):188-93. doi: 10.1016/j.jcv.2007.04.015. Epub 2007 Jun 5. J Clin Virol. 2007. PMID: 17553740

Molecular detection and serotypic characterization of dengue viruses by single-tube multiplex reverse transcriptase-polymerase chain reaction.

Kumaria R, Chakravarti A. Kumaria R, et al. Diagn Microbiol Infect Dis. 2005 Aug;52(4):311-6. doi: 10.1016/j.diagmicrobio.2005.04.013. Diagn Microbiol Infect Dis. 2005. PMID: 15994048

Detection of concurrent infection with multiple dengue virus serotypes in Thai children by ELISA and nested RT-PCR assay.

Chinnawirotpisan P, Mammen MP Jr, Nisalak A, Thaisomboonsuk B, Narupiti S, Thirawuth V, Putnak R, Zhang C. Chinnawirotpisan P, et al. Arch Virol. 2008;153(12):2225-32. doi: 10.1007/s00705-008-0249-9. Epub 2008 Nov 15. Arch Virol. 2008. PMID: 19011729

A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses.

Islam MA, El Zowalaty ME, Islam S, Sharif M, Rahman MR, Amin MR, Ali MM, Rahman MT, Morita K, Ashour HM. Islam MA, et al. Int J Mol Sci. 2020 Nov 5;21(21):8281. doi: 10.3390/ijms21218281. Int J Mol Sci. 2020. PMID: 33167379 Free PMC article.

Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus.

Callahan JD, Wu SJ, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, Porter KR, Murphy GR, Suharyono W, King CC, Hayes CG, Temenak JJ. Callahan JD, et al. J Clin Microbiol. 2001 Nov;39(11):4119-24. doi: 10.1128/JCM.39.11.4119-4124.2001. J Clin Microbiol. 2001. PMID: 11682539 Free PMC article.

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