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Results: 1 to 20 of 287

1.

Covalent immobilization of antibodies on finally inert support surfaces through their surface regions having the highest densities in carboxyl groups.

Batalla P, Fuentes M, Mateo C, Grazu V, Fernandez-Lafuente R, Guisan JM.

Biomacromolecules. 2008 Aug;9(8):2230-6. doi: 10.1021/bm8003594. Epub 2008 Jun 18.

PMID:
18558741
[PubMed - indexed for MEDLINE]
2.

Oriented covalent immobilization of antibodies on physically inert and hydrophilic support surfaces through their glycosidic chains.

Batalla P, Fuentes M, Grazu V, Mateo C, Fernandez-Lafuente R, Guisan JM.

Biomacromolecules. 2008 Feb;9(2):719-23. doi: 10.1021/bm7010906. Epub 2008 Jan 19.

PMID:
18205314
[PubMed - indexed for MEDLINE]
3.

Preparation of inert magnetic nano-particles for the directed immobilization of antibodies.

Fuentes M, Mateo C, Guisán JM, Fernández-Lafuente R.

Biosens Bioelectron. 2005 Jan 15;20(7):1380-7.

PMID:
15590293
[PubMed - indexed for MEDLINE]
4.

Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage.

Mateo C, Fernández-Lorente G, Abian O, Fernández-Lafuente R, Guisán JM.

Biomacromolecules. 2000 Winter;1(4):739-45.

PMID:
11710205
[PubMed - indexed for MEDLINE]
5.

Detection of polyclonal antibody against any area of the protein-antigen using immobilized protein-antigens: the critical role of the immobilization protocol.

Fuentes M, Mateo C, Fernández-Lafuente R, Guisán JM.

Biomacromolecules. 2006 Feb;7(2):540-4.

PMID:
16471927
[PubMed - indexed for MEDLINE]
6.

Oriented covalent immobilization of antibodies onto heterofunctional agarose supports: a highly efficient immuno-affinity chromatography platform.

Batalla P, Bolívar JM, Lopez-Gallego F, Guisan JM.

J Chromatogr A. 2012 Nov 2;1262:56-63. doi: 10.1016/j.chroma.2012.08.058. Epub 2012 Sep 11.

PMID:
23021645
[PubMed - indexed for MEDLINE]
7.

Detection and purification of two antibody-antigen complexes via selective adsorption on lowly activated anion exchangers.

Fuentes M, Pessela BC, Mateo C, Munilla R, Guisán JM, Fernandez-Lafuente R.

J Chromatogr A. 2004 Dec 3;1059(1-2):89-94.

PMID:
15628128
[PubMed - indexed for MEDLINE]
8.

Covalent immobilization of proteins for the biosensor based on imaging ellipsometry.

Wang ZH, Jin G.

J Immunol Methods. 2004 Feb 15;285(2):237-43.

PMID:
14980437
[PubMed - indexed for MEDLINE]
9.

One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports.

Mateo C, Fernández-Lorente G, Cortés E, Garcia JL, Fernández-Lafuente R, Guisan JM.

Biotechnol Bioeng. 2001 Nov;76(3):269-76.

PMID:
11668463
[PubMed - indexed for MEDLINE]
10.

Controlled antibody immobilization onto immunoanalytical platforms by synthetic peptide.

Jung Y, Kang HJ, Lee JM, Jung SO, Yun WS, Chung SJ, Chung BH.

Anal Biochem. 2008 Mar 1;374(1):99-105. Epub 2007 Oct 23.

PMID:
18023402
[PubMed - indexed for MEDLINE]
11.

Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment.

Mateo C, Abian O, Fernández-Lorente G, Pedroche J, Fernández-Lafuente R, Guisan JM, Tam A, Daminati M.

Biotechnol Prog. 2002 May-Jun;18(3):629-34.

PMID:
12052083
[PubMed - indexed for MEDLINE]
12.

Advances in the design of new epoxy supports for enzyme immobilization-stabilization.

Mateo C, Grazú V, Pessela BC, Montes T, Palomo JM, Torres R, López-Gallego F, Fernández-Lafuente R, Guisán JM.

Biochem Soc Trans. 2007 Dec;35(Pt 6):1593-601. Review.

PMID:
18031273
[PubMed - indexed for MEDLINE]
13.

A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from Aspergillus oryzae.

Torres R, Mateo C, Fernández-Lorente G, Ortiz C, Fuentes M, Palomo JM, Guisan JM, Fernández-Lafuente R.

Biotechnol Prog. 2003 May-Jun;19(3):1056-60.

PMID:
12790680
[PubMed - indexed for MEDLINE]
14.

Antibody immobilization onto glow discharge treated polymers.

Safranj A, Kiaei D, Hoffman AS.

Biotechnol Prog. 1991 Mar-Apr;7(2):173-7.

PMID:
1367512
[PubMed - indexed for MEDLINE]
15.

Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties.

Barbosa O, Torres R, Ortiz C, Berenguer-Murcia A, Rodrigues RC, Fernandez-Lafuente R.

Biomacromolecules. 2013 Aug 12;14(8):2433-62. doi: 10.1021/bm400762h. Epub 2013 Jul 16. Review.

PMID:
23822160
[PubMed - indexed for MEDLINE]
16.

Selective adsorption of small proteins on large-pore anion exchangers coated with medium size proteins.

Bolivar JM, Batalla P, Mateo C, Carrascosa AV, Pessela BC, Guisán JM.

Colloids Surf B Biointerfaces. 2010 Jun 15;78(1):140-5. doi: 10.1016/j.colsurfb.2010.02.030. Epub 2010 Mar 6.

PMID:
20307961
[PubMed - indexed for MEDLINE]
17.

Essential role of the concentration of immobilized ligands in affinity chromatography: purification of guanidinobenzoatase on an ionized ligand.

Murza A, Fernández-Lafuente R, Guisán JM.

J Chromatogr B Biomed Sci Appl. 2000 Apr 14;740(2):211-8.

PMID:
10821407
[PubMed - indexed for MEDLINE]
18.

Purification, stabilization, and concentration of very weak protein-protein complexes: Shifting the association equilibrium via complex selective adsorption on lowly activated supports.

Fuentes M, Mateo C, Pessela BC, Guisán JM, Fernandez-Lafuente R.

Proteomics. 2005 Nov;5(16):4062-9.

PMID:
16196088
[PubMed - indexed for MEDLINE]
19.

One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads.

Pessela BC, Mateo C, Carrascosa AV, Vian A, García JL, Rivas G, Alfonso C, Guisan JM, Fernández-Lafuente R.

Biomacromolecules. 2003 Jan-Feb;4(1):107-13.

PMID:
12523854
[PubMed - indexed for MEDLINE]
20.

Immobilization of enzymes on heterofunctional epoxy supports.

Mateo C, Grazu V, Palomo JM, Lopez-Gallego F, Fernandez-Lafuente R, Guisan JM.

Nat Protoc. 2007;2(5):1022-33.

PMID:
17546007
[PubMed - indexed for MEDLINE]

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