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Results: 1 to 20 of 398

1.

Improvement of the purification of Saint Louis encephalitis virus NS2B-NS3 recombinant protease expressed in Escherichia coli.

Pastorino B, Rolland D, Peyrefitte CN, Wurtz N, Almeras L, Bessaud M, Tolou HJ.

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 1;868(1-2):58-63. doi: 10.1016/j.jchromb.2008.04.035. Epub 2008 May 1.

PMID:
18485841
[PubMed - indexed for MEDLINE]
2.

A straightforward experimental approach to expression, purification, refolding, and enzymatic analysis of recombinant dengue virus NS2B(H)-NS3pro protease.

Junaid M, Angsuthanasombat C, Wikberg JE, Ali N, Katzenmeier G.

Biochemistry (Mosc). 2013 Aug;78(8):920-4. doi: 10.1134/S0006297913080099.

PMID:
24228881
[PubMed - indexed for MEDLINE]
Free Article
3.

Catalytically active Dengue virus NS3 protease forms aggregates that are separable by size exclusion chromatography.

Arakaki TL, Fang NX, Fairlie DP, Young PR, Martin JL.

Protein Expr Purif. 2002 Jul;25(2):241-7.

PMID:
12135556
[PubMed - indexed for MEDLINE]
4.

Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro.

Yusof R, Clum S, Wetzel M, Murthy HM, Padmanabhan R.

J Biol Chem. 2000 Apr 7;275(14):9963-9.

PMID:
10744671
[PubMed - indexed for MEDLINE]
Free Article
5.

Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease.

Du GX, Hou LH, Guan RB, Tong YG, Wang HT.

World J Gastroenterol. 2002 Dec;8(6):1088-93.

PMID:
12439931
[PubMed - indexed for MEDLINE]
Free Article
6.

Purification and characterization of the NS3 serine protease domain of hepatitis C virus expressed in Saccharomyces cerevisiae.

Markland W, Petrillo RA, Fitzgibbon M, Fox T, McCarrick R, McQuaid T, Fulghum JR, Chen W, Fleming MA, Thomson JA, Chambers SP.

J Gen Virol. 1997 Jan;78 ( Pt 1):39-43.

PMID:
9010283
[PubMed - indexed for MEDLINE]
Free Article
7.

Expression and purification of recombinant full-length NS3 protease-helicase from a new variant of Hepatitis C virus.

Poliakov A, Hubatsch I, Shuman CF, Stenberg G, Danielson UH.

Protein Expr Purif. 2002 Aug;25(3):363-71.

PMID:
12182815
[PubMed - indexed for MEDLINE]
8.

Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates.

Junaid M, Chalayut C, Sehgelmeble Torrejon A, Angsuthanasombat C, Shutava I, Lapins M, Wikberg JE, Katzenmeier G.

PLoS One. 2012;7(5):e36872. doi: 10.1371/journal.pone.0036872. Epub 2012 May 15.

PMID:
22615830
[PubMed - indexed for MEDLINE]
Free PMC Article
9.

Activation of dengue protease autocleavage at the NS2B-NS3 junction by recombinant NS3 and GST-NS2B fusion proteins.

Wu CF, Wang SH, Sun CM, Hu ST, Syu WJ.

J Virol Methods. 2003 Dec;114(1):45-54.

PMID:
14599678
[PubMed - indexed for MEDLINE]
10.

Steady-state cleavage kinetics for dengue virus type 2 ns2b-ns3(pro) serine protease with synthetic peptides.

Khumthong R, Niyomrattanakit P, Chanprapaph S, Angsuthanasombat C, Panyim S, Katzenmeier G.

Protein Pept Lett. 2003 Feb;10(1):19-26.

PMID:
12625822
[PubMed - indexed for MEDLINE]
11.

Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems.

Khromykh AA, Harvey TJ, Abedinia M, Westaway EG.

J Virol Methods. 1996 Sep;61(1-2):47-58.

PMID:
8882936
[PubMed - indexed for MEDLINE]
12.

A comparative biochemical analysis of the NS2B(H)-NS3pro protease complex from four dengue virus serotypes.

Iempridee T, Thongphung R, Angsuthanasombat C, Katzenmeier G.

Biochim Biophys Acta. 2008 Jul-Aug;1780(7-8):989-94. doi: 10.1016/j.bbagen.2008.03.018. Epub 2008 Apr 7.

PMID:
18439431
[PubMed - indexed for MEDLINE]
Free Article
13.

In vitro determination of dengue virus type 2 NS2B-NS3 protease activity with fluorescent peptide substrates.

Khumthong R, Angsuthanasombat C, Panyim S, Katzenmeier G.

J Biochem Mol Biol. 2002 Mar 31;35(2):206-12.

PMID:
12297031
[PubMed - indexed for MEDLINE]
Free Article
14.

Identification and enzymatic characterization of NS2B-NS3 protease of Alkhurma virus, a class-4 flavivirus.

Bessaud M, Grard G, Peyrefitte CN, Pastorino B, Rolland D, Charrel RN, de Lamballerie X, Tolou HJ.

Virus Res. 2005 Jan;107(1):57-62.

PMID:
15567034
[PubMed - indexed for MEDLINE]
15.

Expression, purification, and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 nonstructural protein 3.

Bartelma G, Padmanabhan R.

Virology. 2002 Jul 20;299(1):122-32.

PMID:
12167347
[PubMed - indexed for MEDLINE]
Free Article
16.
17.

Expression of the Japanese encephalitis virus NS3 and NS2b proteins as glutathione S-transferase fusions.

Shivashankar Y, Satchidanandam V.

Indian J Biochem Biophys. 1995 Dec;32(6):356-60.

PMID:
8714204
[PubMed - indexed for MEDLINE]
18.

Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper.

Lin CW, Lin KH, Lyu PC, Chen WJ.

Virus Res. 2006 Mar;116(1-2):106-13. Epub 2005 Nov 10.

PMID:
16289409
[PubMed - indexed for MEDLINE]
19.

The RNA-unwinding activity of hepatitis C virus non-structural protein 3 (NS3) is positively modulated by its protease domain.

Gu B, Pruss CM, Gates AT, Khandekar SS.

Protein Pept Lett. 2005 May;12(4):315-21.

PMID:
15907174
[PubMed - indexed for MEDLINE]
20.

Functional determinants of NS2B for activation of Japanese encephalitis virus NS3 protease.

Lin CW, Huang HD, Shiu SY, Chen WJ, Tsai MH, Huang SH, Wan L, Lin YJ.

Virus Res. 2007 Jul;127(1):88-94. Epub 2007 Apr 30.

PMID:
17467838
[PubMed - indexed for MEDLINE]

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