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Items: 1 to 20 of 157

1.

Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis.

Queipo-Ortuño MI, De Dios Colmenero J, Macias M, Bravo MJ, Morata P.

Clin Vaccine Immunol. 2008 Feb;15(2):293-6. Epub 2007 Dec 12.

2.

Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis.

Queipo-Ortuño MI, Colmenero JD, Bravo MJ, García-Ordoñez MA, Morata P.

Clin Microbiol Infect. 2008 Dec;14(12):1128-34. doi: 10.1111/j.1469-0691.2008.02095.x.

3.

Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples.

Queipo-Ortuño MI, Colmenero JD, Reguera JM, García-Ordoñez MA, Pachón ME, Gonzalez M, Morata P.

Clin Microbiol Infect. 2005 Sep;11(9):713-8.

4.

Comparison of seven commercial DNA extraction kits for the recovery of Brucella DNA from spiked human serum samples using real-time PCR.

Queipo-Ortuño MI, Tena F, Colmenero JD, Morata P.

Eur J Clin Microbiol Infect Dis. 2008 Feb;27(2):109-14. Epub 2007 Nov 1.

PMID:
17973130
6.

Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.

Al-Soud WA, Jönsson LJ, Râdström P.

J Clin Microbiol. 2000 Jan;38(1):345-50.

7.

Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations.

Surucuoglu S, El S, Ural S, Gazi H, Kurutepe S, Taskiran P, Yurtsever SG.

Pol J Microbiol. 2009;58(1):15-9.

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10.

Real-time PCR for detection of Brucella spp. DNA in human serum samples.

Debeaumont C, Falconnet PA, Maurin M.

Eur J Clin Microbiol Infect Dis. 2005 Dec;24(12):842-5.

PMID:
16341519
11.

Development and evaluation of real-time polymerase chain reaction assays on whole blood and paraffin-embedded tissues for rapid diagnosis of human brucellosis.

Kattar MM, Zalloua PA, Araj GF, Samaha-Kfoury J, Shbaklo H, Kanj SS, Khalife S, Deeb M.

Diagn Microbiol Infect Dis. 2007 Sep;59(1):23-32. Epub 2007 May 29.

PMID:
17532591
12.

Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

Morata P, Queipo-Ortuño MI, de Dios Colmenero J.

J Clin Microbiol. 1998 Sep;36(9):2443-6.

13.

Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR.

Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ.

J Clin Microbiol. 2001 Apr;39(4):1661-4.

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Real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis.

Deféver T, Druet M, Rochelet-Dequaire M, Joannes M, Grossiord C, Limoges B, Marchal D.

J Am Chem Soc. 2009 Aug 19;131(32):11433-41. doi: 10.1021/ja901368m.

PMID:
19722651
16.

[Analysis of PCR-diagnosis of brucellosis in human].

Shestopalov MIu, Kalinovskiĭ AI, Balakhonov SV, Kapustin IuM, Golubinskiĭ EP.

Mol Gen Mikrobiol Virusol. 1999;(3):12-7. Russian.

PMID:
10495977
17.

Autoclave method for rapid preparation of bacterial PCR-template DNA.

Simmon KE, Steadman DD, Durkin S, Baldwin A, Jeffrey WH, Sheridan P, Horton R, Shields MS.

J Microbiol Methods. 2004 Feb;56(2):143-9.

PMID:
14744443
18.

Evaluation of brucellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up.

Maas KS, Méndez M, Zavaleta M, Manrique J, Franco MP, Mulder M, Bonifacio N, Castañeda ML, Chacaltana J, Yagui E, Gilman RH, Guillen A, Blazes DL, Espinosa B, Hall E, Abdoel TH, Smits HL.

Am J Trop Med Hyg. 2007 Apr;76(4):698-702.

19.

[Development of a method for alkaline extraction of DNA from Brucella for diagnosing brucellosis using the polymerase chain reaction].

Shestopalov MIu, Balakhonov SV, Kalinovskiĭ AI, Golubinskiĭ EP.

Mol Gen Mikrobiol Virusol. 1999;(1):30-6. Russian.

PMID:
10190109
20.

Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction.

Elfaki MG, Uz-Zaman T, Al-Hokail AA, Nakeeb SM.

Diagn Microbiol Infect Dis. 2005 Sep;53(1):1-7.

PMID:
16054326
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